Journal ArticleDOI
Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor.
Nicholas M. Gough,Jill Gough,Donald Metcalf,Anne Kelso,Dianne Grail,Nicos A. Nicola,Antony W. Burgess,Ashley R. Dunn +7 more
TLDR
cDNA clones specifying the murine granulocyte–macrophage colony stimulating factor have been isolated and it bears no structural similarity to the functionally related factor, interleukin-3, described recently.Abstract:
cDNA clones specifying the murine granulocyte–macrophage colony stimulating factor have been isolated. This haematopoietic growth factor is encoded by a unique gene specifying a messenger RNA of 1,200 nucleotides and a polypeptide of 118 amino acids. It bears no structural similarity to the functionally related factor, interleukin-3, described recently.read more
Citations
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Journal ArticleDOI
A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation
Gray D. Shaw,Robert Kamen +1 more
TL;DR: It is proposed that the AU sequences are the recognition signal for an mRNA processing pathway which specifically degrades the mRNAs for certain lymphokines, cytokines, and proto-oncogenes.
Journal ArticleDOI
Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs
Carl J. March,Bruce Mosley,Larsen Alf D,Douglas P. Cerretti,Gary Braedt,Virginia L. Price,Steven Gillis,Christopher S. Henney,Shirley R. Kronheim,Kenneth H. Grabstein,Paul J. Conlon,Thomas P. Hopp,David Cosman +12 more
TL;DR: Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-lα and IL-1β), were isolated from a macrophage cDNA library.
Journal ArticleDOI
T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines.
François Fossiez,Odile Djossou,Pascale Chomarat,Leopoldo Flores-Romo,Smina Ait-Yahia,C Maat,Jean-Jacques Pin,Pierre Garrone,Eric Garcia,Sem Saeland,D Blanchard,Claude Gaillard,B Das Mahapatra,E Rouvier,Pierre Golstein,Jacques Banchereau,Serge Lebecque +16 more
TL;DR: Observations suggest that hIL-17 may constitute an early initiator of the T cell-dependent inflammmatory reaction; and an element of the cytokine network that bridges the immune system to hematopoiesis.
Journal ArticleDOI
Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells
Lawrence M. Souza,Thomas C. Boone,Janice Gabrilove,Por H. Lai,Krisztina M. Zsebo,Murdock Douglas C,Vicki R. Chazin,Joan Bruszewski,Hsieng Sen Lu,Kenneth Chen,Jean M. Barendt,Erich Platzer,Malcolm A.S. Moore,Roland Mertelsmann,Karl Welte +14 more
TL;DR: Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities, which has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic cancer.
Journal ArticleDOI
Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins
Gordon G. Wong,JoAnn S. Witek,Patricia A. Temple,Kathleen M. Wilkens,Anne C. Leary,Deborah P. Luxenberg,Simon S. Jones,Eugene L. Brown,Robert M. Kay,Elizabeth C. Orr,Charles B. Shoemaker,David W. Golde,Randal J. Kaufman,Rodney M. Hewick,Elizabeth A. Wang,Steven C. Clark +15 more
TL;DR: Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system and one of these clones was used to produce recombinant GM- CSF in mammalian cells.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
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TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
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Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins
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