Nicolai Krogh

TL;DR: This study constitutes the first comprehensive mapping of 2′-O-Me sites in human rRNA using a high throughput sequencing approach and establishes the existence of a core of constitutively methylated positions and a subset of variable, potentially regulatory positions, and paves the way for experimental analyses of the role of variations in rRNA methylation under different physiological or pathological settings.

TL;DR: A sequencing-based method (RiboMeth-seq) and its application to yeast ribosomes, presently the best-studied eukaryotic model system, enables a detailed evaluation of the importance of RNA modifications in the cells most sophisticated molecular machine.

TL;DR: It is shown that the long isoform of ZAK (ZAKα) directly associates with ribosomes by inserting its flexible C terminus into the ribosomal intersubunit space, and that ablation of the RSR response leads to organismal phenotypes and decreased lifespan in the nematode Caenorhabditis elegans.

TL;DR: This work provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development using a sequencing-based profiling method for ribose methylations.

TL;DR: The results reveal that SIRT7-dependent deacetylation impacts nucleolar activity by an FBL-driven circuitry that mediates cell-cycle-dependent fluctuation of rDNA transcription.