Niek Dekker

TL;DR: A twofold increase in the number of crystallization leads was observed when the proteins were cocrystallized with stabilizing additives as compared with experiments without these additives, suggesting that thermofluor constitutes an efficient generic high-throughput method for identification of protein properties predictive of crystallizability.

TL;DR: The crystal structure of OmpT from Escherichia coli is presented, which shows a 10‐stranded antiparallel β‐barrel that protrudes far from the lipid bilayer into the extracellular space and a novel proteolytic mechanism, involving a His-Asp dyad and an Asp—Asp couple that activate a putative nucleophilic water molecule.

TL;DR: X-ray structures of monomeric and dimeric OMPLA from E. coli provide a detailed view of activation by dimerization of a membrane protein.

TL;DR: Crystal structures of PAR2 in complex with two distinct antagonists and a blocking antibody provide a basis for the development of selective PAR2 antagonists for a range of therapeutic uses.

TL;DR: The first solubilization and purification of a functional GPCR [human adenosine A2A receptor (A2AR)], in the total absence of detergent at any stage, is reported, by exploiting spontaneous encapsulation by styrene maleic acid co-polymer direct from the membrane into a nanoscale SMA lipid particle (SMALP).