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Nobunao Wakabayashi
Researcher at University of Pittsburgh
Publications - 51
Citations - 18539
Nobunao Wakabayashi is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Transcription factor & Signal transduction. The author has an hindex of 36, co-authored 48 publications receiving 16561 citations. Previous affiliations of Nobunao Wakabayashi include Johns Hopkins University & Johns Hopkins University School of Medicine.
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Journal ArticleDOI
Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain
Ken Itoh,Nobunao Wakabayashi,Yasutake Katoh,Tetsuro Ishii,Kazuhiko Igarashi,James Douglas Engel,James Douglas Engel,Masayuki Yamamoto +7 more
TL;DR: It is postulate that Keap1 and Nrf2 constitute a crucial cellular sensor for oxidative stress, and together mediate a key step in the signaling pathway that leads to transcriptional activation by this novel NRF2 nuclear shuttling mechanism.
Journal ArticleDOI
Cell Survival Responses to Environmental Stresses Via the Keap1-Nrf2-ARE Pathway
TL;DR: The development of Nrf2 knockout mice has provided key insights into the toxicological importance of this pathway, and this review highlights the key elements in this adaptive response to protection against acute and chronic cell injury provoked by environmental stresses.
Journal ArticleDOI
Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants
Albena T. Dinkova-Kostova,W. David Holtzclaw,Robert N. Cole,Ken Itoh,Nobunao Wakabayashi,Yasutake Katoh,Masayuki Yamamoto,Paul Talalay +7 more
TL;DR: This finding suggests that reaction of cysteine thiols is followed by rapid formation of protein disulfide linkages, which are the direct sensors of inducers of the phase 2 system.
Journal ArticleDOI
Protection against electrophile and oxidant stress by induction of the phase 2 response: fate of cysteines of the Keap1 sensor modified by inducers.
Nobunao Wakabayashi,Albena T. Dinkova-Kostova,W. David Holtzclaw,Moon Il Kang,Akira Kobayashi,Masayuki Yamamoto,Thomas W. Kensler,Paul Talalay +7 more
TL;DR: Evidence for formation of intermolecular disulfide bridges was obtained by 2D PAGE of extracts of cells treated with inducers, and by the demonstration that whereas C273A and C288A mutants of Keap1 alone could not repress Nrf2 activation of the ARE-luciferase reporter, an equal mixture of these mutant constructs restored repressor activity.
Journal ArticleDOI
Keap1-null mutation leads to postnatal lethality due to constitutive Nrf2 activation
Nobunao Wakabayashi,Ken Itoh,Junko Wakabayashi,Hozumi Motohashi,Shuhei Noda,Satoru Takahashi,Sumihisa Imakado,Tomoe Kotsuji,Fujio Otsuka,Dennis R. Roop,Takanori Harada,James Douglas Engel,Masayuki Yamamoto +12 more
TL;DR: These experiments show that Keap1 acts upstream of Nrf2 in the cellular response to oxidative and xenobiotic stress, and breeding to NRF2-deficient mice reversed the phenotypic Keap 1 deficiencies.