Norbert Schülke

TL;DR: In this article, a matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) was used to identify the functional epitope on human immunodeficiency virus envelope glycoprotein gp41 for the broadly neutralizing anti-gp41 human monoclonal antibody 2F5.

TL;DR: The application of Blue Native polyacrylamide gel electrophoresis (BN-PAGE), a high-resolution molecular sizing method, to the study of viral envelope proteins has relevance for rational vaccine design.

TL;DR: This disulfide-shackled virus is a unique tool with potential utility in vaccine design, drug discovery, and elucidation of the HIV-1 entry process, suggesting that downstream fusion events and structures figure prominently in the host immune response.

TL;DR: The data suggests that Env-VLP sera were primarily focused on nonfunctional forms of Env on VLP surfaces, possibly gp120/gp41 monomers and not the trimers, which suggests that to make progress toward a more effective VLP-based vaccine, it will need to find ways to refocus the attention of B cells on native trimers.

TL;DR: In this article, the authors report on the antibody responses generated in rabbits against a soluble, cleaved, trimeric form of HIV-1 JR-FL Env. Neutralization of these viruses was immunoglobulin mediated and was predominantly caused by antibodies to gp120 epitopes.