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Benevides C. C. Pessela
Researcher at Spanish National Research Council
Publications - 92
Citations - 3563
Benevides C. C. Pessela is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Immobilized enzyme & Lipase. The author has an hindex of 30, co-authored 88 publications receiving 3253 citations. Previous affiliations of Benevides C. C. Pessela include Instituto Politécnico Nacional.
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Journal ArticleDOI
Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins
Cesar Mateo,Jose M. Palomo,Manuel Fuentes,Lorena Betancor,Valeria Grazú,Fernando López-Gallego,Benevides C. C. Pessela,Aurelio Hidalgo,Gloria Fernández-Lorente,Roberto Fernandez-Lafuente,Jose M. Guisan +10 more
TL;DR: Very active and highly stabilized derivatives of many enzymes and proteins have been prepared using activated supports that immobilize proteins, via a two-point reaction involving the region/s of the protein surface with the higher densities of amino groups.
Journal ArticleDOI
Some special features of glyoxyl supports to immobilize proteins
Cesar Mateo,Olga Abian,Marieta Bernedo,Emma Cuenca,Manuel Fuentes,Gloria Fernández-Lorente,Jose M. Palomo,Valeria Grazú,Benevides C. C. Pessela,Cecilia Giacomini,Cecilia Giacomini,Gabriela Irazoqui,Gabriela Irazoqui,Andrea Villarino,Andrea Villarino,Karen Ovsejevi,Karen Ovsejevi,Francisco Batista-Viera,Roberto Fernandez-Lafuente,Jose M. Guisan +19 more
TL;DR: This mechanism promotes that proteins become immobilized by the area/s where the highest lysine residues density is located and explain the high enzyme stabilization usually achieved by using this immobilization technique.
Journal ArticleDOI
Epoxy-Amino Groups: A New Tool for Improved Immobilization of Proteins by the Epoxy Method
Cesar Mateo,Rodrigo Torres,Gloria Fernández-Lorente,Claudia Ortiz,Manuel Fuentes,Aurelio Hidalgo,Fernando López-Gallego,Olga Abian,Jose M. Palomo,Lorena Betancor,Benevides C. C. Pessela,Jose M. Guisan,Roberto Fernandez-Lafuente +12 more
TL;DR: Stability of the immobilized enzyme has been found to be much higher using the new support than in preparations using the conventional ones in many cases, and immobilization is much more rapid using amino-epoxy supports than employing conventional epoxy supports.
Journal ArticleDOI
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
Cesar Mateo,Valeria Grazú,Benevides C. C. Pessela,Tamara Montes,Jose M. Palomo,Rodrigo Torres,Fernando López-Gallego,Roberto Fernandez-Lafuente,Jose M. Guisan +8 more
TL;DR: The possibilities of a directed immobilization of mutated enzymes (change of an amino acid by cysteine on specific points of the protein surface) on tailor-made disulfide-epoxy supports will be discussed as an almost-ideal procedure to achieve very intense and very efficient rigidification of a desired region of industrial enzymes.
Journal ArticleDOI
The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition: Complete hydrolysis of lactose in dairy products
Benevides C. C. Pessela,Cesar Mateo,Manuel Fuentes,Alejandro Vian,José Luis García,Alfonso V. Carrascosa,Jose M. Guisan,Roberto Fernandez-Lafuente +7 more
TL;DR: In this paper, the β-galactosidase from Thermus sp. T2 (Htag-BgaA) was shown to be competitively inhibited by galactose (3.1 mM) and non-competitively by glucose (49.9 mM).