Pablo Aleza

TL;DR: This work sequence and compare citrus genomes—a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes—and shows that cultivated types derive from two progenitor species.

TL;DR: A reference genetic map of citrus, used to facilitate the chromosome assembly of the first citrus reference genome sequence, was established and confirmed that Clementine arose from hybridization between ‘Mediterranean’ mandarin and sweet orange.

TL;DR: Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.

TL;DR: This work describes a new methodology based on in vitro shoot- Tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry.

TL;DR: This work describes an effective methodology to recover triploid hybrids from 2x × 2x hybridisations based on in vitro embryo rescue and ploidy level determination by means of flow cytometry and the influence of parents and environmental conditions has been analysed.