P
Paula Veríssimo
Researcher at University of Coimbra
Publications - 42
Citations - 1363
Paula Veríssimo is an academic researcher from University of Coimbra. The author has contributed to research in topics: Support vector machine & Proteases. The author has an hindex of 19, co-authored 40 publications receiving 1255 citations. Previous affiliations of Paula Veríssimo include Oklahoma Medical Research Foundation & Universidade Nova de Lisboa.
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Journal ArticleDOI
Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L.
TL;DR: The results herein described on the structural and kinetic properties of the cardosins indicate that they are the products of distinct genes which have probably arisen by gene duplication.
Journal ArticleDOI
The vegetable rennet of Cynara cardunculus L. contains two proteinases with chymosin and pepsin-like specificities
TL;DR: The enzyme composition of cardoon rennet closely resembles that of calf rennet, and in terms of specificity and kinetic parametersCardosin A is similar to chymosin whereas cardosin B is similarto pepsin.
Journal ArticleDOI
Pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides.
Raquel Vinhas,Luísa Cortes,Inês Cardoso,Vera M. Mendes,Bruno Manadas,Ana Todo-Bom,Euclides Pires,Paula Veríssimo +7 more
TL;DR: This work has shown that pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides through pathways related to EMT and “cell reprograming”.
Journal ArticleDOI
Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of Cynara cardunculus L.
Carlos Frazão,Isabel Bento,Júlia Costa,Cláudio M. Soares,Paula Veríssimo,Carlos Faro,Euclides Pires,Jon Cooper,Maria Arménia Carrondo +8 more
TL;DR: Based on the crystal structure, a possible mechanism whereby cardosin A might be orientated at the cell surface of the style to interact with its putative receptor from pollen is proposed.
Journal ArticleDOI
Cardosin A, an abundant aspartic proteinase, accumulates in protein storage vacuoles in the stigmatic papillae of Cynara cardunculus L.
Miguel Ramalho-Santos,José Pissarra,Paula Veríssimo,Susana Pereira,Roberto Salema,Euclides Pires,Carlos Faro +6 more
TL;DR: It is shown that expression of cardosin A is highly restricted to the pistils, and that the enzyme accumulates mainly in protein storage vacuoles of the stigmatic papillae, as well as in flower senescence.