Journal ArticleDOI
Pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides.
Raquel Vinhas,Luísa Cortes,Inês Cardoso,Vera M. Mendes,Bruno Manadas,Ana Todo-Bom,Euclides Pires,Paula Veríssimo +7 more
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TLDR
This work has shown that pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides through pathways related to EMT and “cell reprograming”.Abstract:
To cite this article: Vinhas R, Cortes L, Cardoso I, Mendes VM, Manadas B, Todo-Bom A, Pires E, Verissimo P. Pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides. Allergy 2011; 66: 1088–1098.
Abstract
Background: Allergic disorders, such as seasonal rhinitis and asthma, are increasing causes of morbidity worldwide and often result from exposure to airborne pollen. Pollen allergy has a remarkable clinical impact all over Europe. In fact, epidemiological longitudinal studies confirm that pollen species usually considered with low allergenic potential became more recently responsible for intense allergic reactions. In this study, we aimed to characterize major pollen proteolytic activity and evaluate its contribution to the immunologic and inflammatory response to airborne allergens.
Methods: Proteolytic activity in four pollen diffusates with distinct allergenicity, Olea europaea, Dactylis glomerata, Cupressus sempervirens and Pinus sylvestris, was evaluated through several enzymatic assays. The action of pollen proteases on the paracellular integrity of Calu-3, grown at the air–liquid interphase, was evaluated through a transepithelial permeability assay. Immunoblot and immunofluorescence experiments were performed to analyse the disruption of intercellular complexes. Degradation of bioactive peptides by pollen crude extracts was assessed by mass spectrometry.
Results: All pollen diffusates were shown to have high molecular weight proteases with serine and/or aminopeptidase activity. These proteases increased Calu-3 transepithelial permeability through disruption of transmembrane adhesion proteins: occludin, claudin-1 and E-cadherin. Moreover, they were able to degrade airway bioactive peptides and were not blocked by endogenous protease inhibitors.
Conclusion: Pollen grains with distinct allergenic abilities release proteases that might be involved in the sensitization to a range of airborne allergens by facilitating allergen delivery across the epithelium and also contribute directly to the inflammation characteristic of allergic diseases.read more
Citations
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Journal ArticleDOI
Epithelial barrier function: At the front line of asthma immunology and allergic airway inflammation
Steve N. Georas,Fariba Rezaee +1 more
TL;DR: The structure and function of epithelial apical junctional complexes are reviewed, emphasizing how regulation of the epithelial barrier affects innate and adaptive immunity.
Journal ArticleDOI
Barrier function of airway tract epithelium
TL;DR: In this paper, the authors reviewed the regulation of components of barrier function with respect to chronic airway diseases and showed that impairment of one or more of these essential components may increase susceptibility to infection and promote exaggerated and prolonged innate immune responses to environmental factors including allergens and pathogens resulting in chronic inflammation.
Journal ArticleDOI
Origin and Consequences of Necroinflammation.
TL;DR: A novel classification of DAMPs is introduced in this review to help detect the relative contribution of each RN-pathway to certain physiological and pathophysiological conditions of necroinflammation.
Journal ArticleDOI
Danger-Associated Molecular Patterns (DAMPs): the Derivatives and Triggers of Inflammation.
TL;DR: The limited number of recognized DAMPs are only a fraction of the vast array of other DAMPs, and efforts should be focused on the elimination of inflammatory agents.
Journal ArticleDOI
Environmental factors in epithelial barrier dysfunction.
Zeynep Çelebi Sözener,Zeynep Çelebi Sözener,Lacin Cevhertas,Lacin Cevhertas,Kari C. Nadeau,Mübeccel Akdis,Cezmi A. Akdis +6 more
TL;DR: The aim of this article was to discuss the effects of environmental factors such as protease enzymes of allergens, detergents, tobacco, ozone, particulate matter, diesel exhaust, nanoparticles, and microplastic on the integrity of the epithelial barriers in the context of epithelial barrier hypothesis.
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