P
Pauline Schaap
Researcher at University of Dundee
Publications - 148
Citations - 5266
Pauline Schaap is an academic researcher from University of Dundee. The author has contributed to research in topics: Dictyostelium discoideum & Dictyostelium. The author has an hindex of 41, co-authored 141 publications receiving 4851 citations. Previous affiliations of Pauline Schaap include University of Groningen & Leiden University.
Papers
More filters
Journal ArticleDOI
Lithium ions induce prestalk-associated gene expression and inhibit prespore gene expression in Dictyostelium discoideum
Dorien J.M. Peters,M.M. van Lookeren Campagne,P. J. M. Van Haastert,Wouter Spek,Pauline Schaap +4 more
TL;DR: Results indicate that the induction of the cysteine proteinase-2 gene by Li+ is not due to elevated levels of Ins(1,4,5)P3, and it is, however, possible that inhibition of prespore gene expression by Li+.
Journal ArticleDOI
Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium.
TL;DR: A series of expression vectors for purification of native proteins and protein complexes in Dictyostelium were constructed and all members of the Arp2/3 complex could be identified.
Journal ArticleDOI
cAMP-induced desensitization of surface cAMP receptors in Dictyostelium: different second messengers mediate receptor phosphorylation, loss of ligand binding, degradation of receptor, and reduction of receptor mRNA levels.
TL;DR: The cAMP analogue (Rp)-cAMPS induced loss of ligand binding without inducing second messenger responses or phosphorylation, redistribution, and degradation of the receptor implies that desensitization is composed of multiple components that are regulated by different but partly overlapping sensory transduction pathways.
Journal ArticleDOI
Temperature‐sensitive Gβ mutants discriminate between G protein‐dependent and ‐independent signaling mediated by serpentine receptors
TL;DR: It is shown that Gβ is directly required for adenylyl cyclase A activation and for morphogenetic signaling during the entire developmental program.
Journal ArticleDOI
Cytoplasmic acidification facilitates but does not mediate DIF-induced prestalk gene expression in Dictyostelium discoideum
TL;DR: The results suggest that the effects of DIF on gene expression are regulated by intracellular pH, but do not support a role for protons as direct intermediates in the DIF signal transduction pathway.