It is shown that, particularly for terrestrial cellular telephony, the interference-suppression feature of CDMA (code division multiple access) can result in a many-fold increase in capacity over analog and even over competing digital techniques. A single-cell system, such as a hubbed satellite network, is addressed, and the basic expression for capacity is developed. The corresponding expressions for a multiple-cell system are derived. and the distribution on the number of users supportable per cell is determined. It is concluded that properly augmented and power-controlled multiple-cell CDMA promises a quantum increase in current cellular capacity. >

On the capacity of a cellular CDMA system

中枢神経系疾患の治療は正常細胞（ニューロン）の機能維持を目的とするが，脳血管障害のように機能障害の原因が細胞の死滅に基づくことは多い．一方，脳腫瘍の治療においては薬物療法や放射線療法といった腫瘍細胞の死滅を目標とするものが大きな位置を占める．いずれの場合にも，細胞死の機序を理解することは各種病態や治療法の理解のうえで重要である．現在のところ最も研究の進んでいる細胞死の型はアポトーシスである．そのなかで重要な位置を占めるミトコンドリアにおける反応および抗アポトーシス因子について概要を紹介する．

Neuroscience 細胞死：最近の知見

Schizophrenia is a heritable brain illness with unknown pathogenic mechanisms. Schizophrenia's strongest genetic association at a population level involves variation in the major histocompatibility complex (MHC) locus, but the genes and molecular mechanisms accounting for this have been challenging to identify. Here we show that this association arises in part from many structurally diverse alleles of the complement component 4 (C4) genes. We found that these alleles generated widely varying levels of C4A and C4B expression in the brain, with each common C4 allele associating with schizophrenia in proportion to its tendency to generate greater expression of C4A. Human C4 protein localized to neuronal synapses, dendrites, axons, and cell bodies. In mice, C4 mediated synapse elimination during postnatal development. These results implicate excessive complement activity in the development of schizophrenia and may help explain the reduced numbers of synapses in the brains of individuals with schizophrenia.

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Schizophrenia risk from complex variation of complement component 4

저작근 근전도에 관한 정상교합자와 ii급 부정교합자의 비교 연구

Our understanding of the cellular implementation of systems-level neural processes like action, thought and emotion has been limited by the availability of tools to interrogate specific classes of neural cells within intact, living brain tissue. Here we identify and develop an archaeal light-driven chloride pump (NpHR) from Natronomonas pharaonis for temporally precise optical inhibition of neural activity. NpHR allows either knockout of single action potentials, or sustained blockade of spiking. NpHR is compatible with ChR2, the previous optical excitation technology we have described, in that the two opposing probes operate at similar light powers but with well-separated action spectra. NpHR, like ChR2, functions in mammals without exogenous cofactors, and the two probes can be integrated with calcium imaging in mammalian brain tissue for bidirectional optical modulation and readout of neural activity. Likewise, NpHR and ChR2 can be targeted together to Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally. NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.

Multimodal fast optical interrogation of neural circuitry

Helix Command Specific 2 (HCS2) gene is constantly expressed in parietal premotor (command) interneurons involved in control of the terrestrial snail Helix lucorum withdrawal behavior as a trigger element. It is also expressed under noxious conditions in other neurons presumably involved in withdrawal behavior. In this study we addressed the role of neuropeptide CNP4, encoded by gene HCS2, in the regulation of activity of the respiratory system, and in the influence on growth of isolated neurons in culture. It was shown that activity of the premotor interneuron elicits a direct effect (pneumostome closure), and a delayed intensification of respiratory movements. Application of CNP4 mimicked the delayed effects. Presence of the peptide CNP4 in solution for cultured neurons led to increase of neuronal growth. Immunochemical localization of the protein precursor encoded by gene HCS2 and peptide CNP4 in the cultured premotor interneurons revealed their preferential presence in the growth cones. The obtained results suggest that CNP4 may be secreted and involved in synergic regulation of behavior of a snail.

Functional role of neuropeptide CNP4 encoded by gene HCS2 in Helix lucorum nervous system

Analysis of our own data and of the results from other groups brings about a concept concerning community of the basic molecular mechanisms involved in neuroplasticity and neuropathology at different levels. Along with the fundamental significance of this idea for understanding of processes taking place both in normal brain and in neuropathological conditions, the concept is of principal importance for practical application. Failure in development of the "pathogenetically directed" approaches to treatment of neural diseases (e. g. stroke) are, in particular, related to the neglecting of identity of the basic molecular mechanism underlying both normal brain functioning and neuropathological conditions resulting in intervention into these common mechanisms (NMDA receptors blockade, inhibitor of the so called "apoptotic" proteases).

[Commonality of molecular mechanisms of neuroplasticity and neuropathology: integrative approach].

Studies have been made of the effect of GABA on the activity of identified neurones of the central nervous system of the snail Helix lucorum. GABA perfusion within first 10 min resulted in the increase of reactions to intracellular and transsynaptic stimulation of the neurones studied, depolarization by 3-4 mV being also observed. After this period, further perfusion decreased the spontaneous activity and neuronal reaction, this effect being accompanied by the decrease in membrane resistance. Synaptic and axonal conduction remained unaffected, although cells responded by the action potentials only to strong stimulation. After 30 min of GABA perfusion, tactile stimulation, which in the intact animals induces the avoidance reaction led to opening of the respiratory orifice, i.e. a component of the respiratory cycle.

Effect of gamma aminobutyric acid on command neurons of Helix lucorum snail neurons

Thermogenetics appeared recently as an evolutionary advance in methods of optical stimulation of nerve cells and uses focused light to activate light-sensitive cation channels in the neuron membrane. Light activation and opening of light-sensitive channels, which can be expressed genetically in any type of neuron, induces neuron membrane depolarization and a resultant action potential. In contrast to classical methods of optogenetics, which use the visible spectrum, thermogenetics uses channels sensitive to warming. This provides the opportunity for additional activation of these channels not only using IR light, but also other methods of warming nervous tissue, such as ultrasound or microwave radiation. The penetrability of living tissue to stimulation with IR radiation is an order of magnitude greater than that for visible spectrum radiation, which allows thermogenetics methods to be used in vivo without invasive surgical methods to “clear the way” for optogenetic stimulation. On the other hand, the thermal nature of the stimulation imposes additional limits on use of the method, as heating must be sufficiently gentle so as not to induce heat shock at the cellular level, and the threshold of activation must be sufficiently high for channel opening not to occur spontaneously at normal physiological temperatures.

Thermogenetics as a New Direction in Controlling the Activity of Neural Networks

The morphology of cells immunoreactive to an antibody against molluscan insulin-related peptide (MIP-IR) was studied in two species of terrestrial snail: Helix lucorum L. and Eobania vermiculata L. Immunocytochemical staining with this antibody to MIP revealed 100-130 cells in the postcerebrum, located in two clusters with common pathways in the dorsal body nerve and the cerebral artery nerve. About 75% of the MIP-IR cells were labeled by backfilling of the dorsal body nerve in Helix and Eobania; the corresponding figures for labeling by backfilling of the cerebral artery nerve were about 60% in Helix and 30% in Eobania. Upon intracellular staining of neurons of the dorsomedial postcerebrum, where most of the MIP-IR cells were located, it was found that they projected either in the dorsal body nerve or in the cerebral artery nerve or in both. The obtained data suggest that growth and reproduction processes (both functions were attributed to the insulin-related peptide-containing neurons) are regulated by the two, partially coinciding subsets of postcerebral MIP-IR neurons with different types of branching.

Pavel M. Balaban

Papers

Functional role of neuropeptide CNP4 encoded by gene HCS2 in Helix lucorum nervous system

[Commonality of molecular mechanisms of neuroplasticity and neuropathology: integrative approach].

Effect of gamma aminobutyric acid on command neurons of Helix lucorum snail neurons

Thermogenetics as a New Direction in Controlling the Activity of Neural Networks

Morphological basis for coordination of growth and reproduction processes in the CNS of two terrestrial snails