Peter Schreier

TL;DR: The discovery of the secreted protein gene clusters and the functional demonstration of their decisive role in the infection process illuminate previously unknown mechanisms of pathogenicity operating in biotrophic fungi.

TL;DR: In this paper, the chimaeric DNA sequence is used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chamaeric precursor then transports the heterology in vivo into a chloroplast of a cell.

TL;DR: A stilbene synthase gene unit which comprises approximately 6,700 base pairs and exhibits 3 EcoRI, 3 HindIII and 1 PstI cleavage sites, and which can be obtained by partial cleavage of the plasmid PGS 828.1 using EcoRI and HindIII, or from PLASMID 828,1 using SstI and PvuII as discussed by the authors.

TL;DR: Analysis of an ozone inducible STS transcript and its corresponding promoter (Vst1), combined with the β-glucuronidase (GUS) reporter gene, showed that the Vst1 promoter was induced in palisade and spongy parenchyma cells and to a lesser extent in epidermal cells.

TL;DR: The isolation of secreted proteins using a signal sequence trap approach with bioinformatic analyses and the subsequent characterization of knock-out mutants indicates that Hum3 and Rsp1 are pathogenicity proteins that share an essential function in early stages of the infection.