Peter Steinlein

TL;DR: A profile of repressive histone lysine methylation states for the repetitive complement of four distinct mouse epigenomes is defined and tandem repeats and dsRNA are suggested as primary triggers for more stable chromatin imprints.

TL;DR: Application of the small particles in more concentrated form and over extended periods of time improves transfection activity, and analysis of gene expression at the cellular level revealed that the differences in overall gene expression largely result from different intensities per expressing cell, while the difference in the percentage of expressing cells is less substantial.

TL;DR: It is shown here that transferrin-polylysine andtransferrin-protamine, when complexed to plasmid DNA containing a luciferase reporter gene, is efficiently bound and moved into avian erythroblasts by endocytosis.

TL;DR: The impact of the GR on erythropoiesis in vivo is analyzed, using GR-deficient mice or mice expressing a GR defective for transactivation to determine whether a particular, GR-sensitive progenitor could be identified as being responsible for the stress response.

TL;DR: Receptor-mediated uptake of ligand-containing DNA/PEI (molecular weight, 800 kd) complexes was found to occur quickly (within 1 hour), whereas unspecific uptake through adsorptive endocytosis is less efficient or requires extended periods to reach the same degree of internalization.