P
Piotr Bobrowicz
Researcher at Merck & Co.
Publications - 11
Citations - 715
Piotr Bobrowicz is an academic researcher from Merck & Co.. The author has contributed to research in topics: Pichia pastoris & Glycan. The author has an hindex of 7, co-authored 11 publications receiving 650 citations.
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Journal ArticleDOI
The Saccharomyces cerevisiae ACR3 gene encodes a putative membrane protein involved in arsenite transport.
TL;DR: The results suggest that arsenite resistance in yeast is mediated by an arsenite transporter (Acr3p), which has hydrophobic character with 10 putative transmembrane spans and is probably located in yeast plasma membrane.
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Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae
TL;DR: Findings suggest that all three novel genes: ACR1, ACR2 and ACR3 are involved in the arsenical‐resistance phenomenon in S. cerevisiae.
Journal ArticleDOI
Identification of a New Family of Genes Involved in β-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans
Céline Mille,Piotr Bobrowicz,Pierre-André Trinel,Huijuan Li,Emmanuel Maes,Yann Guérardel,Chantal Fradin,María Martínez-Esparza,Robert C. Davidson,Guilhem Janbon,Daniel Poulain,Stefan Wildt +11 more
TL;DR: A family of four related new genes in P. pastoris and subsequently nine homologs in C. albicans are identified, cloning, and characterization of a novel family of fungal genes involved in β-mannose transfer are presented.
Journal ArticleDOI
Production of sialylated O-linked glycans in Pichia pastoris.
Stephen R. Hamilton,W. James Cook,Sujatha Gomathinayagam,Irina Burnina,John Bukowski,Daniel Hopkins,Shaina Schwartz,Min Du,Nathan J Sharkey,Piotr Bobrowicz,Stefan Wildt,Huijuan Li,Terrance A. Stadheim,Juergen H. Nett +13 more
TL;DR: This work describes a method for the co-expression of an α-1,2-mannosidase, which reduces these glycans to primarily a single O-linked mannose residue, and extends the utility of the P. pastoris production platform beyond N-linked glycosylated biotherapeutics to include molecules possessing O- linked glycans.
Journal ArticleDOI
Characterization of the Pichia pastoris protein-O-mannosyltransferase gene family.
Juergen H. Nett,W. James Cook,Ming-Tang Chen,Robert C. Davidson,Piotr Bobrowicz,Warren Kett,Elena E. Brevnova,Thomas I. Potgieter,Mark Mellon,Bianka Prinz,Byung-Kwon Choi,Dongxing Zha,Irina Burnina,John Bukowski,Min Du,Stefan Wildt,Stephen R. Hamilton +16 more
TL;DR: This report identifies and characterize the members of the P .