Journal ArticleDOI
Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae
Reads0
Chats0
TLDR
Findings suggest that all three novel genes: ACR1, ACR2 and ACR3 are involved in the arsenical‐resistance phenomenon in S. cerevisiae.Abstract:
A 4.2 kb region from Saccharomyces cerevisiae chromosome XVI was isolated as a yeast fragment conferring resistance to 7 mM-sodium arsenite (NaAsO2), when put on a multicopy plasmid. Homology searches revealed a cluster of three new open reading frames named ACR1, ACR2 and ACR3. The hypothetical projuct of the ACR1 gene is similar to the transcriptional regulatory proteins, encoded by YAP1, and YAP2 genes from S. cerevisiae. Disruption of the ACR1 gene conduces to an arsenite and arsenate hypersensitivity phenotype. The ACR2 gene is indispensable for arsenate but not for arsenite resistance. The hypothetical product of the ACR3 gene shows high similarity to the hypothetical membrane protein encoded by Bacillus subtilis ORF1 of the skin element and weak similarity to the ArsB membrane protein of the Staphylococcus aureus arsenical-resistance operon. Overexpression of the ACR3 gene confers an arsenite- but not an arsenate-resistance phenotype. The presence of ACR3 together with ACR2 on a multicopy plasmid expands the resistance phenotype into arsenate. These findings suggest that all three novel genes: ACR1, ACR2 and ACR3 are involved in the arsenical-resistance phenomenon in S. cerevisiae. (C) 1997 by John Wiley & Sons, Ltd.read more
Citations
More filters
Journal ArticleDOI
Heavy Metal Tolerance in Plants: Role of Transcriptomics, Proteomics, Metabolomics, and Ionomics.
TL;DR: This review summarizes various tolerance strategies of plants under heavy metal toxicity covering the role of metabolites (metabolomics), trace elements (ionomics), transcription factors (transcriptomics), various stress-inducible proteins (proteomics) as well as therole of plant hormones.
Journal ArticleDOI
Biochemistry of arsenic detoxification
TL;DR: While the overall schemes for arsenic resistance are similar in prokaryotes and eukaryotes, some of the specific proteins are the products of separate evolutionary pathways.
Journal ArticleDOI
Microbial arsenic: from geocycles to genes and enzymes.
TL;DR: The DNA sequencing and protein crystal structures have established the convergent evolution of three classes of arsenate reductases, which involve three cysteine thiols and S-As bond intermediates, so convergence evolution to similar mechanisms has taken place.
Journal ArticleDOI
Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance
TL;DR: Studies on genotoxicity of metals discussed in this review showed that genotoxic effects could be in part responsible for metal phytotoxicity, deserving further examination to elucidate the underlying mechanisms.
Journal ArticleDOI
Protein tyrosine phosphatases: mechanisms of catalysis and regulation.
John M. Denu,Jack E. Dixon +1 more
TL;DR: It is shown that in two instances, regulation of phosphatase activity employs autoinhibitory mechanisms involving either intermolecular or intramolecular interactions, whereby inhibition is mediated by sterically blocking the active-site cleft.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Book ChapterDOI
One-step gene disruption in yeast
TL;DR: The one-step gene disruption techniques described here are versatile in that a disruption can be made simply by the appropriate cloning experiment and the resultant chromosomal insertion is nonreverting and contains a genetically linked marker.