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Piotr Chylenski

Researcher at Norwegian University of Life Sciences

Publications -  12
Citations -  916

Piotr Chylenski is an academic researcher from Norwegian University of Life Sciences. The author has contributed to research in topics: Cellulase & Lignocellulosic biomass. The author has an hindex of 9, co-authored 11 publications receiving 594 citations.

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Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2.

TL;DR: The use of H2O2 by a monocopper enzyme that is otherwise cofactor-free offers new perspectives regarding the mode of action of copper enzymes, and these findings have implications for the enzymatic conversion of biomass in Nature and in industrial biorefining.
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The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

TL;DR: The present report shows that the use of small amounts of a liquid bulk chemical, H2O2, provides an alternative to the currently available processes, which likely is cheaper and more easy to control, while giving higher product yields.
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Lytic Polysaccharide Monooxygenases in Enzymatic Processing of Lignocellulosic Biomass

TL;DR: The discovery of lytic polysaccharide monooxygenases (LPMOs) has revolutionized enzymatic processing ofpolysaccharides, in particular, recalcitrant insoluble polysacchides, such as cellulose.
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Methylation of the N-terminal histidine protects a lytic polysaccharide monooxygenase from auto-oxidative inactivation.

TL;DR: An in‐depth functional comparison of two variants of a family AA9 LPMO from Thermoascus aurantiacus showed that the methylated variant of TaLPMO9A was more resistant to excess H2O2 and showed better process performance when using conditions that promote generation of reactive‐oxygen species.
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Development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass

TL;DR: The development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI™ process, which is based on sulfite pulping technology are presented.