Qinlong Zhu

TL;DR: A robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants and provides examples of loss-of-function gene mutations in T0 rice and Arabidopsis plants.

TL;DR: An overview of current advances on applications of this technology in plants is presented, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing theinduced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISpr/ Cas9 system in plants.

TL;DR: Use of the clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) and Cpf1 systems in plants involves many steps, including the selection of appropriate specific target site(s) that should have no highly homologous sequences as the potential off-target sites in the genome.

TL;DR: The results provide the first evidence that plant genomes have an executor R gene family of which members execute their function and spectrum of disease resistance by recognizing the cognate TALEs in the pathogen.

TL;DR: A high-efficiency vector system for transgene stacking is developed and used to engineer anthocyanin biosynthesis in rice endosperm, suggesting the potential utility of this toolkit for synthetic biology and improvement of agronomic traits in plants.