Yuanling Chen

TL;DR: A robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants and provides examples of loss-of-function gene mutations in T0 rice and Arabidopsis plants.

TL;DR: It is shown in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide and plays an additional role in promoting the editing of atp 6 mRNAs, independent of its cleavage function.

TL;DR: In this paper, a new mitochondrial gene, WA352, which originated recently in wild rice, confers the wild abortive male sterility (CMS-WA) because the protein it encodes interacts with the nuclear-encoded mitochondrial protein COX11.

TL;DR: An overview of current advances on applications of this technology in plants is presented, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing theinduced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISpr/ Cas9 system in plants.

TL;DR: It is shown that a locus for indica-japonica hybrid male sterility, Sa, comprises two adjacent genes, SaM and SaF, encoding a small ubiquitin-like modifier E3 ligase-like protein and an F-box protein, respectively.