R
Randolph S. Ashton
Researcher at University of Wisconsin-Madison
Publications - 51
Citations - 2331
Randolph S. Ashton is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Induced pluripotent stem cell & Neural stem cell. The author has an hindex of 21, co-authored 46 publications receiving 1958 citations. Previous affiliations of Randolph S. Ashton include Wisconsin Institutes for Discovery & Helen Wills Neuroscience Institute.
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Journal ArticleDOI
The Influence of Hydrogel Modulus on the Proliferation and Differentiation of Encapsulated Neural Stem Cells
Akhilesh Banerjee,Manish Arha,Soumitra Choudhary,Randolph S. Ashton,Surita R. Bhatia,David V. Schaffer,Ravi S. Kane +6 more
TL;DR: This work describes studies of the proliferation and differentiation of neural stem cells encapsulated within three-dimensional scaffolds--alginate hydrogels--whose elastic moduli were varied over two orders of magnitude.
Journal ArticleDOI
Astrocytes regulate adult hippocampal neurogenesis through ephrin-B signaling
Randolph S. Ashton,Anthony Conway,Chinmay Pangarkar,Jamie M. Bergen,Kwang-il Lim,Kwang-il Lim,Priya S. Shah,Mina J. Bissell,David V. Schaffer +8 more
TL;DR: Ephrin-B2+ astrocytes promote neuronal differentiation of adult NSCs through juxtacrine signaling, findings that advance the understanding of adult neurogenesis and may have future regenerative medicine implications.
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Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture.
TL;DR: A method for creating alginate hydrogels with adjustable degradation rates that can be used as scaffolds for stem cells and it is demonstrated that neural progenitor cells (NPCs) can be cultured and expanded in vitro in this degradable alginated hydrogel system.
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Deterministic HOX Patterning in Human Pluripotent Stem Cell-Derived Neuroectoderm
Ethan S. Lippmann,Clay E. Williams,David A. Ruhl,Maria C. Estevez-Silva,Edwin R. Chapman,Joshua J. Coon,Randolph S. Ashton +6 more
TL;DR: It is demonstrated that successive activation of fibroblast growth factor, Wnt/β-catenin, and growth differentiation factor signaling during hPSC differentiation generates stable, homogenous SOX2+/Brachyury+ neuromesoderm that exhibits progressive, full colinear HOX activation over 7 days.
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A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.
Mohsen Afshar Bakooshli,Ethan S. Lippmann,Ben Mulcahy,Nisha Iyer,Christine T Nguyen,Kayee Tung,Bryan A. Stewart,Hubrecht van den Dorpel,Hubrecht van den Dorpel,Tobias Fuehrmann,Molly S. Shoichet,Anne Bigot,Elena Pegoraro,Henry Ahn,Henry Ahn,Howard J. Ginsberg,Howard J. Ginsberg,Mei Zhen,Mei Zhen,Randolph S. Ashton,Penney M. Gilbert +20 more
TL;DR: A three-dimensional co-culture method whereby human muscle progenitors mixed with human pluripotent stem cell-derived motor neurons self-organize to form functional NMJ connections is described, offering a simple method to model and evaluate adult human NMJ de novo development or disease in culture.