R
Robert A. Sikkink
Researcher at Mayo Clinic
Publications - 18
Citations - 867
Robert A. Sikkink is an academic researcher from Mayo Clinic. The author has contributed to research in topics: Calcineurin & Binding site. The author has an hindex of 13, co-authored 18 publications receiving 833 citations. Previous affiliations of Robert A. Sikkink include University of Texas Southwestern Medical Center & University of Rochester.
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Journal ArticleDOI
Identification of Gene Mutations in Autosomal Dominant Polycystic Kidney Disease through Targeted Resequencing
Sandro Rossetti,Katharina Hopp,Robert A. Sikkink,Jamie L. Sundsbak,Yean Kit Lee,Vickie J. Kubly,Bruce W. Eckloff,Christopher J. Ward,Christopher G. Winearls,Vicente E. Torres,Peter C. Harris +10 more
TL;DR: A strategy to analyze both the PKD1 and PKD2 genes using next-generation sequencing by pooling long-range PCR amplicons and multiplexing bar-coded libraries is developed and validated and is a model for future genetic characterization of large ADPKD populations.
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Failure of Calcineurin Inhibitors to Prevent Pressure-Overload Left Ventricular Hypertrophy in Rats
TL;DR: In this article, the authors showed that calcineurin inhibitors, cyclosporin A (CsA) and FK 506 (0.3 mg · kg −1 · d −1 ) or even higher doses of CsA (10 and 20 mg· kg − 1 · d−1 ) were sufficient to prevent the development of pressure-overload hypertrophy in the spontaneously hypertensive rat and aortic banding.
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Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein.
TL;DR: It is reported that calcineurin is also the target of a recently identified Ca2+-binding protein, CHP (forcalcineur in homologous protein), which shares a high degree of homology with the regulatory B subunit of calcinesurin and with calmodulin.
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Neelaredoxin, an Iron-binding Protein from the Syphilis Spirochete, Treponema pallidum, Is a Superoxide Reductase
Tijana Jovanović,Carla Ascenso,Karsten R. O. Hazlett,Robert A. Sikkink,Carsten Krebs,Robert D. Litwiller,Linda M. Benson,Isabel Moura,José J. G. Moura,Justin D. Radolf,Boi Hanh Huynh,Stephen Naylor,Frank Rusnak +12 more
TL;DR: This finding, the first description of a T. pallidum iron-binding protein, indicates that the syphilis spirochete copes with oxidative stress via a primitive mechanism, which, thus far, has not been described in pathogenic bacteria.
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Mate Pair Sequencing of Whole-Genome-Amplified DNA Following Laser Capture Microdissection of Prostate Cancer
Stephen J. Murphy,John C. Cheville,Shabnam Zarei,Sarah H. Johnson,Robert A. Sikkink,Farhad Kosari,Andrew L. Feldman,Bruce W. Eckloff,R. Jeffrey Karnes,George Vasmatzis +9 more
TL;DR: A novel methodology to partner laser capture microdissection (LCM) with sequencing platforms, through a whole-genome amplification (WGA) protocol performed in situ directly on LCM engrafted cells, aiding in the derivation of genomic aberrations that initiate cancer and drive cancer progression is presented.