Roberto Galizi

TL;DR: Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci meets the minimum requirement for a gene drive targeting female reproduction in an insect population, which could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.

TL;DR: It is found that CRISPR–Cas9-targeted disruption of the intron 4–exon 5 boundary aimed at blocking the formation of functional AgdsxF did not affect male development or fertility, whereas females homozygous for the disrupted allele showed an intersex phenotype and complete sterility.

TL;DR: It is demonstrated that distorter male mosquitoes can efficiently suppress caged wild-type mosquito populations, providing the foundation for a new class of genetic vector control strategies.

TL;DR: These findings represent the first documented example of selection for resistance to a synthetic gene drive and lead to important design recommendations and considerations in order to mitigate for resistance in future gene drive applications.

TL;DR: A CRISPR-Cas9 sex distortion system that targets ribosomal sequences restricted to the member species of the Anopheles gambiae complex is described that is broadly applicable for the species-specific control of any pest or vector species with an XY sex-determination system by targeting sequences exclusive to the female sex chromosome.