Showing papers by "Rodney Harris published in 1982"
TL;DR: The difference in outcome between FS and US mothers is significant, and the most likely explanation is that supplementation has prevented some neural tube defects, but further studies are needed.
Abstract: An earlier preliminary paper is expanded. Women who had given birth to one or more infants with a neural tube defect were recruited into a trial of periconceptional vitamin supplementation. Two hundred mothers attending five centres were fully supplemented (FS), 50 were partially supplemented (PS), and 300 were unsupplemented (US). Neural tube defect recurrences in the study pregnancies were 1(0.5%), in FS, none in PS, and 13 (4%) in US mothers. The difference in outcome between FS and US mothers is significant. The most likely explanation is that supplementation has prevented some neural tube defects, but further studies are needed.
144 citations
TL;DR: Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy using an osmium impregnation technique, which eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens.
Abstract: Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens. Chromosomes were of three-dimensional cylindrical profile, with well-defined chromatids and centromeres. Prior to Giemsa-banding a smooth surface morphology was observed. Relaxation of chromosome integrity by Giemsa-banding pretreatment allowed resolution of several orders of chromosome structure not previously demonstrated by scanning electron microscopy. The observed organization of the chromatin fibres allowed parallels to be drawn with the radial loop model of chromosome construction as described by Marsden and Laemmli.
68 citations
TL;DR: The qualitative acetylcholinesterase (AChE) test has been used prospectively in conjunction with χ‐fetoprotein (AFP) assay on 986 amniotic fluid specimens received in this laboratory during 1980, but AChE gives occasional false positives so that it cannot be relied on in isolation for the diagnosis of neural‐tube defects.
17 citations
2 citations