Journal ArticleDOI
High-resolution scanning electron microscopy of human metaphase chromosomes
TLDR
Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy using an osmium impregnation technique, which eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens.Abstract:
Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens. Chromosomes were of three-dimensional cylindrical profile, with well-defined chromatids and centromeres. Prior to Giemsa-banding a smooth surface morphology was observed. Relaxation of chromosome integrity by Giemsa-banding pretreatment allowed resolution of several orders of chromosome structure not previously demonstrated by scanning electron microscopy. The observed organization of the chromatin fibres allowed parallels to be drawn with the radial loop model of chromosome construction as described by Marsden and Laemmli.read more
Citations
More filters
Journal ArticleDOI
High-density three-dimensional localization microscopy across large volumes
Wesley R. Legant,Lin Shao,Jonathan B. Grimm,Timothy A. Brown,Daniel E. Milkie,Brian B. Avants,Luke D. Lavis,Eric Betzig +7 more
TL;DR: Lattice light-sheet microscopy is combined with newly developed, freely diffusing, cell-permeable chemical probes with targeted affinity for DNA, intracellular membranes or the plasma membrane to perform high–localization precision, ultrahigh–labeling density, multicolor localization microscopy in samples up to 20 μm thick.
Journal ArticleDOI
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organization
TL;DR: The results suggest that the pathway of chromatin condensation through mitosis consists of concurrent changes occurring at several levels of Chromatin organization, rather than a strictly sequential folding process.
Journal ArticleDOI
Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery
Daniel G Booth,Masatoshi Takagi,Luis Sanchez-Pulido,E Petfalski,Giulia Vargiu,Kumiko Samejima,Naoko Imamoto,Chris P. Ponting,David Tollervey,William C. Earnshaw,Paola Vagnarelli +10 more
TL;DR: In this paper, the authors report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells, which is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of B23/nucleophosmin.
Journal ArticleDOI
Computer simulations predict that chromosome movements and rotations accelerate mitotic spindle assembly without compromising accuracy.
TL;DR: Computer simulations and high-resolution microscopy are used to test plausible pathways of spindle assembly in realistic geometry and suggest that an optimal combination of centrosomal and chromosomal pathways, spatially biased microtubule growth, and chromosome movements and rotations is needed to complete prometaphase in 10–20 min while keeping erroneous merotelic attachments down to a few percent.
Journal ArticleDOI
The fragile X: a scanning electron microscope study.
TL;DR: SEM has allowed a more precise location of the fragile site to the Xq27 .
Related Papers (5)
Metaphase chromosome structure: Evidence for a radial loop model
M.P.F. Marsden,Ulrich K. Laemmli +1 more