R
Roland Silber
Publications - 6
Citations - 520
Roland Silber is an academic researcher. The author has contributed to research in topics: Capripoxvirus & Lumpy skin disease virus. The author has an hindex of 6, co-authored 6 publications receiving 443 citations.
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Journal ArticleDOI
Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxviruses
Charles Euloge Lamien,Mamadou Lelenta,Wilfried Goger,Roland Silber,Eeva S.M. Tuppurainen,Mirta Matijevic,Antony George Luckins,Adama Diallo +7 more
TL;DR: A species-specific, real time PCR assay based on unique molecular markers that were found in the G-protein-coupled chemokine receptor (GPCR) gene sequences of CaPVs, that uses dual hybridization probes for their simultaneous detection, quantitation and genotyping.
Journal ArticleDOI
Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination.
Christian Le Goff,Charles Euloge Lamien,Emna Fakhfakh,Amélie Chadeyras,Elexpeter Aba-Adulugba,Geneviève Libeau,Eeva S.M. Tuppurainen,David B. Wallace,Tajelser Adam,Roland Silber,Velý Gulyaz,Hafsa Madani,Philippe Caufour,Salah Hammami,Adama Diallo,Emmanuel Albina +15 more
TL;DR: The suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses is described and unique features such as a longer N-terminal end with a proximal hydrophobic alpha-helix and a shorter serine-rich C-tail are revealed.
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Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus.
Charles Euloge Lamien,Christian Le Goff,Roland Silber,David B. Wallace,Velý Gulyaz,Eeva S.M. Tuppurainen,Hafsa Madani,Philippe Caufour,Tajelser Adam,Mehdi El Harrak,Antony George Luckins,Emmanuel Albina,Adama Diallo +12 more
TL;DR: In this article, the authors used RPO30-based classical PCR test to differentiate SPPV from GTPV and Lumpy skin disease virus (LSDV) in small ruminants.
Journal ArticleDOI
Monkey CV1 cell line expressing the sheep-goat SLAM protein: a highly sensitive cell line for the isolation of peste des petits ruminants virus from pathological specimens.
Caroline Mélanie Adombi,Mamadou Lelenta,Charles Euloge Lamien,David Shamaki,Yao Mathurin Koffi,Abdallah Traoré,Roland Silber,Emmanuel Couacy-Hymann,Sanne Charles Bodjo,Joseph Allico Djaman,Antony George Luckins,Adama Diallo +11 more
TL;DR: It is demonstrated that monkey CV1 cells expressing goat SLAM are also highly efficient for isolating PPRV from pathological samples, suggesting that SLAM, as is in the case for MV, CDV and RPV, is also a receptor for PPRv.
Journal ArticleDOI
Development of a cost-effective method for capripoxvirus genotyping using snapback primer and dsDNA intercalating dye
Esayas Gelaye,Esayas Gelaye,Esayas Gelaye,Charles Euloge Lamien,Roland Silber,Eeva S.M. Tuppurainen,Reingard Grabherr,Adama Diallo +7 more
TL;DR: The design and analytical performances of a new molecular assay for CaPV genotyping using unlabelled snapback primers in the presence of dsDNA intercalating EvaGreen dye are described, able to simultaneously detect and genotype CaPVs in 63 samples with a sensitivity and specificity of 100%.