Rossukon Thongwichian

TL;DR: The previously uncharacterized NMR properties of lysine propionylation, butyrylation, succinylation, malonylation and crotonylation are delineated to define an initial reference frame for comprehensive PTM studies by high-resolution NMR spectroscopy.

TL;DR: Phosphorylation is quantitatively monitored with consecutive 2D 1H-15N band-selective optimized-flip-angle short-transient (SOFAST)-heteronuclear multiple-quantum (HMQC) NMR experiments, which provide atomic-resolution insights into the phosphorylation levels of individual substrate residues and time-dependent changes thereof, thereby offering unique advantages over western blotting and mass spectrometry.

TL;DR: Results obtained in this study highlight the complementary nature of antibody- and NMR-based phospho-detection techniques and exemplify the usefulness of time-resolved NMR measurements in providing fast and quantitative readouts of kinase activities and kinase inhibitor efficacies in native cellular environments.

TL;DR: An NMR-based reporter approach is introduced to directly identify active kinases and phosphatases in complex physiological environments such as cell lysates and to measure their individual activities in a semicontinuous fashion.

TL;DR: This chapter describes the preparation of oocyte in-cell NMR samples for IDP studies in Xenopus laevis oocytes, the first eukaryotic cellular model system to be established.