R
Ruchi Sharma
Researcher at National Institutes of Health
Publications - 68
Citations - 1138
Ruchi Sharma is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Retinal pigment epithelium & Induced pluripotent stem cell. The author has an hindex of 14, co-authored 43 publications receiving 760 citations. Previous affiliations of Ruchi Sharma include National Dairy Research Institute & Post Graduate Institute of Medical Education and Research.
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Journal ArticleDOI
Clinical-grade stem cell-derived retinal pigment epithelium patch rescues retinal degeneration in rodents and pigs
Ruchi Sharma,Vladimir Khristov,Aaron Rising,Balendu Shekhar Jha,Roba Dejene,Nathan Hotaling,Yichao Li,Jonathan Stoddard,Casey Stankewicz,Qin Wan,Connie Zhang,Mercedes Campos,Kiyoharu J. Miyagishima,David M. McGaughey,Rafael Villasmil,Mary J. Mattapallil,Boris V. Stanzel,Haohua Qian,Wai T. Wong,Lucas Chase,Steve Charles,Trevor J. McGill,Sheldon S. Miller,Arvydas Maminishkis,Juan Amaral,Kapil Bharti +25 more
TL;DR: The results suggest that the in vitro and in vivo preclinical functional validation of iPSC-RPE patches developed here might ultimately be useful for evaluation and optimization of autologous i PSC-based therapies.
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VEGF regulates local inhibitory complement proteins in the eye and kidney
Lindsay S. Keir,Rachel Firth,Lyndsey Aponik,Daniel Feitelberg,Susumu Sakimoto,Edith Aguilar,Gavin I. Welsh,Anna Richards,Yoshihiko Usui,Simon C. Satchell,Valeryia Kuzmuk,Richard J M Coward,Jonathan Goult,Katherine R. Bull,Ruchi Sharma,Kapil Bharti,Peter D. Westenskow,Iacovos P. Michael,Moin A. Saleem,Martin Friedlander +19 more
TL;DR: Results suggest that VEGF protects the retinal and glomerular microvasculature, not only through VEGFR2-mediated vasculotrophism, but also through modulation of local complement proteins that could protect against complement-mediated damage.
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Primary Cilium-Mediated Retinal Pigment Epithelium Maturation Is Disrupted in Ciliopathy Patient Cells
Helen May-Simera,Qin Wan,Balendu Shekhar Jha,Juliet Hartford,Vladimir Khristov,Roba Dejene,Justin Chang,Sarita Rani Patnaik,Quanlong Lu,Poulomi Banerjee,Jason S. Silver,Christine Insinna-Kettenhofen,Dishita Patel,Mostafa Reza Lotfi,May Christine V. Malicdan,Nathan Hotaling,Arvydas Maminishkis,Rupa Sridharan,Brian P. Brooks,Kiyoharu J. Miyagishima,Meral Gunay-Aygun,Rajarshi Pal,Christopher J. Westlake,Sheldon S. Miller,Ruchi Sharma,Kapil Bharti +25 more
TL;DR: It is demonstrated that the retinal pigment epithelium (RPE) requires a functional primary cilium for complete maturation and that RPE maturation defects in ciliopathies precede photoreceptor degeneration, and a developmental role for primary cilia in epithelial maturation is demonstrated.
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In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications
Kiyoharu J. Miyagishima,Qin Wan,Barbara Corneo,Ruchi Sharma,Mostafa Reza Lotfi,Nathan C. Boles,Fang Hua,Arvydas Maminishkis,Congxiao Zhang,Timothy A. Blenkinsop,Vladimir Khristov,Balendu Shekhar Jha,Omar Memon,Sunita L. D’Souza,Sally Temple,Sheldon S. Miller,Kapil Bharti +16 more
TL;DR: The donor and clonal variation was characterized and found that iPSC‐RPE function was more significantly affected by the genetic differences between different donors than the epigenetic differences associated with different starting tissues.
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Biallelic Mutations in MITF Cause Coloboma, Osteopetrosis, Microphthalmia, Macrocephaly, Albinism, and Deafness
Aman George,Dina J. Zand,Robert B. Hufnagel,Ruchi Sharma,Yuri V. Sergeev,Janet M. Legare,Gregory M. Rice,Jessica Scott Schwoerer,Mariana Rius,Laura H. Tetri,David M. Gamm,Kapil Bharti,Brian P. Brooks,Brian P. Brooks +13 more
TL;DR: A syndrome that is characterized by coloboma, osteopetrosis, microphthalmia, macrocephaly, albinism, and deafness is described, which suggests a role for MITF in regulating processes such as optic-fissure closure and bone development or homeostasis, which go beyond what is usually seen in individuals carrying monoallelic MITF mutations.