Studies of the molecular genetics, mechanisms of activation and functional connections between general phenylpropanoid metabolism and certain branch pathways in parsley, bean, potato plants and cell cultures

Physiology and Molecular Biology of Phenylpropanoid Metabolism

The chalcone synthase superfamily of type III polyketide synthases.

Resveratrol: a molecule whose time has come? And gone?

BIOCHEMISTRY OF POL YKETIDE SYNTHESIS: STRUCTURE OF THE SYNTHASES. . . . . . .. . . . . . . . . . . . . . .. . . . . .. . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . 40 THE GENES FOR FATTY ACID AND POLYKETIDE BIOSyNTHESIS. . . . . . . . . ...... 44 Fatty Acid Biosynthesis in Escherichia coli. . . . . . . .. . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . .. . .. . . . . . 44 Polyketide Antibiotic Biosynthesis by Actinomycetes. . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Putative Polyketides Involved in Nodulation by Rhizobium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Anthocyanin and Phytoalexin Biosynthesis in Flowering Plants. . . . . . . . . . . . . . . . . . . . . . . . . 49 MOLECULAR GENETICS OF FATTY ACID AND POLYKETIDE BIOSYNTHESIS. . . . . . . . . . . . . . . . ... . . . .. . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . ... . . . . . . 50 Vertebrate Type I FAS Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . 50 The Fungal Type I FAS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 The Type I PKS of Penicillium patulum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 The Type II FAS of E. coli . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . . . . . . . . . . . . . 53 Type II Plant FAS Systems .... . . . . . . . . . . . . . . ....... . . . . . . . . .. . . . . . . . . . . . . ... . . . . . . . . . . . . . . . . . . . 54 Type 11 PKS Systems in Streptomyces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SS Macrolide Antibiotic Biosynthesis and the Module Hypothesis . . . . . . . . . . . . . . . . . . . . . . . . . . 57 Plant Chalcone and Resveratrol Synthases . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . 58 SPECIFICITY OF FAS AND PKS SYSTEMS: THE PROBLEM OF .. PROGRAMMING". . . . . . . . . . . . . . . . ... . .. . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . .. 59

Molecular Genetics of Polyketides and Its Comparison to Fatty Acid Biosynthesis

Phytoalexins: enzymology and molecular biology.

Flavanone synthase from irradiated cell suspension cultures of parsley was purified to apparent homogeneity. Molecular weights of about 77000 for the enzyme and about 42000 for the subunits were determined respectively by sedimentation-equilibrium measurements and disc-gel electrophoresis in the presence of dodecyl sulfate. A specific antiserum was prepared for the enzyme and was used in an assay for flavanone synthase mRNA activity in partially purified RNA preparations. The apparent molecular size of flavanone synthase mRNA was estimated by sucrose gradient centrifugation and gel electrophoresis under partially denaturing conditions. Values of about 17 S and Mr= 0.62 × 106 were obtained. The fractionation patterns suggested that flavanone synthase mRNA was homogeneous in size. All together, the results support the idea that the enzyme is composed of two subunits which are probably identical.



Amino acid analysis and a microbial assay were carried out to test the possible occurrence of cysteamine, β-alanine, and pantothenate in the enzyme. The results were negative, indicating the absence of pantetheine or a similar residue. The possible similarity in mechanism between flavanone synthase and 3-oxoacyl-(acyl carrier protein) synthase is discussed.

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1979.tb13235.x

Flavanone Synthase from Petroselinum hortense

Electron microscopic studies on the foetal fibrin clot

Amino acid sequence of yeast proteinase B inhibitor 1 comparison with inhibitor 2.

Cleavage specificity of the serine proteinase from rat liver mitochondria

/pdf/molecular-characterization-of-human-foetal-fibrinogen-4yf5ocibo3.pdf

Rudolf Tesch

Papers

Flavanone Synthase from Petroselinum hortense

Electron microscopic studies on the foetal fibrin clot

Amino acid sequence of yeast proteinase B inhibitor 1 comparison with inhibitor 2.

Cleavage specificity of the serine proteinase from rat liver mitochondria

Molecular Characterization of Human Foetal Fibrinogen