R
Ryan C. Kunz
Researcher at Harvard University
Publications - 32
Citations - 1661
Ryan C. Kunz is an academic researcher from Harvard University. The author has contributed to research in topics: Active site & Phosphorylation. The author has an hindex of 19, co-authored 32 publications receiving 1410 citations. Previous affiliations of Ryan C. Kunz include Lawrence Berkeley National Laboratory & University of Nebraska–Lincoln.
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Journal ArticleDOI
The membrane-associated methane monooxygenase (pMMO) and pMMO-NADH:quinone oxidoreductase complex from Methylococcus capsulatus bath
Dong W. Choi,Ryan C. Kunz,Eric S. Boyd,Jeremy D. Semrau,William E. Antholine,Jong-In Han,James A. Zahn,James A. Zahn,Jeffrey M. Boyd,Arlene de la Mora,Alan A. DiSpirito +10 more
TL;DR: It is demonstrated that copper not only regulates the metabolic switch between the two methane monooxygenases but also regulates the level of expression of the pMMO and the development of internal membranes.
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S-Nitrosylation links obesity-associated inflammation to endoplasmic reticulum dysfunction
Ling Yang,Ediz S. Calay,Jason Fan,Alessandro Arduini,Ryan C. Kunz,Steven P. Gygi,Abdullah Yalcin,Suneng Fu,Gökhan S. Hotamisligil,Gökhan S. Hotamisligil +9 more
TL;DR: It is shown that, in the setting of obesity, inflammatory input through increased inducible nitric oxide synthase (iNOS) activity causes S-nitrosylation of a key UPR regulator, IRE1α, which leads to a progressive decline in hepatic I RE1α-mediated XBP1 splicing activity in both genetic and dietary models of obesity.
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Regulation of meiotic recombination via Mek1-mediated Rad54 phosphorylation
Hengyao Niu,Lihong Wan,Valeria Busygina,Youngho Kwon,Jasmina A. Allen,Xue Li,Ryan C. Kunz,Kazuishi Kubota,Beatrice Wang,Patrick Sung,Kevan M. Shokat,Steven P. Gygi,Nancy M. Hollingsworth +12 more
TL;DR: Mek1 phosphorylation provides a dynamic means of controlling recombination partner choice in meiosis in two ways: (1) it reduces Rad51 activity through inhibition of Rad51/Rad54 complex formation, and (2) it suppresses Rad51-mediated strand invasion of sister chromatids via a Rad54-independent mechanism.
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Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/orbitrap mass spectrometer
Brian K. Erickson,Mark P. Jedrychowski,Graeme C. McAlister,Robert A. Everley,Ryan C. Kunz,Steven P. Gygi +5 more
TL;DR: It is found that ratio distortion remained a problem for phosphopeptide analysis in multiplexed quantitative workflows, but the underlying cause of interference may not be due to coeluting and cofragmented peptides but instead from consistent, low level background fragmentation.
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Sensitive multiplexed analysis of kinase activities and activity-based kinase identification
Kazuishi Kubota,Rana Anjum,Rana Anjum,Yonghao Yu,Ryan C. Kunz,Jannik N. Andersen,Manfred Kraus,Heike Keilhack,Kumiko Nagashima,Stefan Krauss,Cloud P. Paweletz,Ronald C. Hendrickson,Adam S. Feldman,Chin-Lee Wu,John Rush,Judit Villén,Steven P. Gygi +16 more
TL;DR: An improved single-reaction strategy, which quantifies the phosphorylation of 90 synthetic peptides in a single mass spectrometry run, is compatible with nanogram to microgram amounts of cell lysate and enhances kinase monospecificity through substrate competition effects.