S.J. Flint

TL;DR: The concentrations, in copies per cell, of viral RNA sequences complementary to different regions of the genome were determined at 8, 18 and 32 hours after infection of human cells with adenovirus type 2 to account for the inability to detect, by filter hybridization, certain classes of early mRNA sequences during the late stage of infection.

TL;DR: Specific fragments of Adenov virus 5 DNA produced by digestion of intact, 32P-labeled viral DNA with restriction endonucleases Eco R1 and and Hpa 1 are shown to be expressed as mRNA during the early phase of an Adenovirus 5 lytic infection.

TL;DR: The time course of synthesis of single-stranded viral DNA sequences relative to accumulation of total viral DNA during the productive cycle and their behavior following a shift of H5ts125-infected cells in which viral DNA replication has begun from a permissive to a nonpermissive temperature support the contention that these sequences are indeed generated as adenovirus DNA is replicated.

TL;DR: The separated strands of fragments of 32P-labeled Adenov virus 2 DNA generated by the restriction endonuclease Bam Hl have been used to improve the resolution of maps of both Adenovirus 2 and Adenov Virus 5 “late” mRNA, which are described here.

TL;DR: All the late adenovirus 5 temperature-sensitive mutants synthesized normal wild-type levels of late cytoplasmic RNA at the nonpermissive temperature, except ts2, which appears to overproduce certain cytopLasmic species.