Sayda El Safi

TL;DR: SSCP had advantages over the other methods employed for investigation of sequence variation within the species L. donovani and confirmed that SSCP was able to detect genetic diversity at the level of a single nucleotide.

TL;DR: Accuracy of rapid diagnostic tests was high in the Indian subcontinent; however, in Brazilian and East African samples, reduced sensitivity suggests that several cannot be used alone to exclude visceral leishmaniasis.

TL;DR: It is argued that the implementation of clinical bacteriology in low-resource settings improves patient management, provides valuable surveillance for local antibiotic treatment guidelines and national policies, and supports containment of antimicrobial resistance and the prevention and control of hospital-acquired infections.

TL;DR: Evaluation of rapid diagnostic tests for confirmation of VL disease finds that PCR is usually highly sensitive for detection of leishmanial infection, but this does not mean PCR will be useful for the confirmation of acute Vl disease in patients in endemic areas, as many carriers of the infection in these areas will be PCRpositive without developing VL Disease.

TL;DR: A generic, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay, for the identification of Leishmania species from clinical samples, and the potential to use LAMP as a diagnostic tool for leishmaniasis is discussed.