Sergey M. Vorobiev

TL;DR: It is estimated that the new method should allow rapid structure determination without experimental phase information for over half the cases where current methods fail, given diffraction data sets of better than 3.2 Å resolution, four or fewer copies in the asymmetric unit, and the availability of structures of homologous proteins with >20% sequence identity.

TL;DR: In this paper, structural and biochemical studies of the Xanthomonas campestris TDO and a related protein SO4414 from Shewanella oneidensis were performed.

TL;DR: This work aims to demonstrate the efforts towards in-situ applicability of x-ray diffraction-spot assisted, 3D image analysis to provide real-time information about the response of the immune system to x-rays.

TL;DR: UNC119 is a Gα subunit cofactor essential for G protein trafficking in sensory cilia, thereby providing a stable UNC119–Tα-GTP complex capable of diffusing from the inner segment back to the outer segment after light-induced translocation.

TL;DR: Kinetic experiments indicate that the active site cysteines are rapidly acylated as programmed by design, but the subsequent slow hydrolysis of the acyl-enzyme intermediate limits overall catalytic efficiency, which highlights the challenges that computational design must overcome to achieve high levels of activity.