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Shi Jian Ding
Researcher at Environmental Molecular Sciences Laboratory
Publications - 5
Citations - 217
Shi Jian Ding is an academic researcher from Environmental Molecular Sciences Laboratory. The author has contributed to research in topics: Phosphoproteomics & Proteomics. The author has an hindex of 5, co-authored 5 publications receiving 210 citations. Previous affiliations of Shi Jian Ding include Pacific Northwest National Laboratory & University of Nebraska Medical Center.
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Journal ArticleDOI
Profiling signaling polarity in chemotactic cells
Yingchun Wang,Shi Jian Ding,Wei Wang,Jon M. Jacobs,Wei-Jun Qian,Ronald J. Moore,Feng Yang,David G. Camp,Richard D. Smith,Richard L. Klemke +9 more
TL;DR: This work applies global proteome profiling in combination with newly developed quantitative phosphoproteomics approaches for comparative analysis of the cell body (CB) and PD proteome of chemotactic cells, revealing networks of signaling proteins that partition to the PD and/or the CB compartments.
Journal ArticleDOI
An Extensive Survey of Tyrosine Phosphorylation Revealing New Sites in Human Mammary Epithelial Cells
Tyler H. Heibeck,Shi Jian Ding,Lee K. Opresko,Rui Zhao,Athena A. Schepmoes,Feng Yang,Aleksey V. Tolmachev,Matthew E. Monroe,David G. Camp,Richard D. Smith,H. Steven Wiley,Wei-Jun Qian +11 more
TL;DR: An extensive survey of tyrosine phosphorylation sites in a normal-derived human mammary epithelial cell (HMEC) line by applying antiphosphotyrosine peptide immunoaffinity purification coupled with high sensitivity capillary liquid chromatography tandem mass spectrometry revealed that a majority of highly phosphorylated proteins were relatively low-abundance.
Journal ArticleDOI
Quantitative proteomic approaches for studying phosphotyrosine signaling.
TL;DR: Recent advances in analytical methodologies for enrichment, identification and accurate quantitation of tyrosine-phosphorylated proteins and peptides are outlined, as well as non-mass spectrometry-based methods, such as those using protein/peptide arrays.
Journal ArticleDOI
Applying a targeted label-free approach using LC-MS AMT tags to evaluate changes in protein phosphorylation following phosphatase inhibition.
Feng Yang,Navdeep Jaitly,Hemalatha Jayachandran,Quanzhou Luo,Matthew E. Monroe,Xiuxia Du,Marina A. Gritsenko,Rui Zhang,David J. Anderson,Samuel O. Purvine,Joshua N. Adkins,Ronald J. Moore,Heather M. Mottaz,Shi Jian Ding,Mary S. Lipton,David G. Camp,Harold R. Udseth,Richard D. Smith,Sandra Rossie +18 more
TL;DR: To identify phosphoproteins regulated by the phosphoprotein phosphatase (PPP) family of S/T phosphatases, a large-scale characterization of changes in protein phosphorylation on extracts from HeLa cells treated with or without calyculin A, a potent PPP enzyme inhibitor was performed.
Journal ArticleDOI
Quantitative phosphoproteome analysis of lysophosphatidic acid induced chemotaxis applying dual-step (18)O labeling coupled with immobilized metal-ion affinity chromatography.
Shi Jian Ding,Yingchun Wang,Jon M. Jacobs,Wei-Jun Qian,Feng Yang,Aleksey V. Tolmachev,Xiuxia Du,Wei Wang,Ronald J. Moore,Matthew E. Monroe,Samuel O. Purvine,Katrina M. Waters,Tyler H. Heibeck,Joshua N. Adkins,David G. Camp,Richard L. Klemke,Richard Smith +16 more
TL;DR: An integrated quantitative phosphoproteomics approach and its application for comparative analysis of Cos-7 cells in response to lysophosphatidic acid (LPA) gradient stimulation is presented.