Sooyeon Lee

TL;DR: It is shown that macroautophagy requires the Alzheimer's disease-related protein presenilin-1 (PS1) for v-ATPase targeting to lysosomes, lysOSome acidification, and proteolysis during autophagy, which represents a basis for pathogenic protein accumulations and neuronal cell death in AD.

TL;DR: It is shown that constitutive macroautophagy in primary cortical neurons is highly efficient, because newly formed autophagosomes are rapidly cleared by fusion with lysosomes, accounting for their scarcity in the healthy brain, and that the autophagic pathology observed in AD most likely arises from impaired clearance of AVs rather than strong autophagy induction alone.

TL;DR: The AD-associated defects in neuronal lysosomal proteolysis are identified as a possible basis for the selective transport abnormalities and highly characteristic pattern of neuritic dystrophy associated with AD.

TL;DR: The most commonly used pH probes for sensitivity and localisation are evaluated, and LysoSensor yellow/blue‐dextran, among currently used probes, are identified as having the optimal profile of properties for measuring lysosomal pH.

TL;DR: This work shows that hind limb intramuscular injection of αS can induce pathology in the CNS in the human Ala53Thr (M83) and wild-type (M20) αS transgenic mouse models, and is the first evidence that an entire neurodegenerative proteinopathy associated with a robust, lethal motor phenotype can be initiated by peripheral inoculation with a pathogenic protein.