S
Sriram Kosuri
Researcher at University of California, Los Angeles
Publications - 73
Citations - 7794
Sriram Kosuri is an academic researcher from University of California, Los Angeles. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 25, co-authored 70 publications receiving 6572 citations. Previous affiliations of Sriram Kosuri include University of California, Berkeley & Harvard University.
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Journal ArticleDOI
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Prashant Mali,John Aach,P. Benjamin Stranges,Kevin M. Esvelt,Mark Moosburner,Sriram Kosuri,Luhan Yang,George M. Church,George M. Church +8 more
TL;DR: This system is engineer to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).
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Next-Generation Digital Information Storage in DNA
TL;DR: This work developed a strategy to encode arbitrary digital information in DNA, wrote a 5.27-megabit book using DNA microchips, and read the book by using next-generation DNA sequencing.
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Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription
Feng Zhang,Le Cong,Le Cong,Simona Lodato,Sriram Kosuri,Sriram Kosuri,George M. Church,George M. Church,Paola Arlotta +8 more
TL;DR: 17 TALEs are synthesized that are customized to recognize specific DNA-binding sites, and it is demonstrated that they can specifically modulate transcription of endogenous genes (SOX2 and KLF4) in human cells.
Journal ArticleDOI
Large-scale de novo DNA synthesis: technologies and applications
Sriram Kosuri,George M. Church +1 more
TL;DR: Methods and caveats for the de novo synthesis of DNA are summarized, with particular emphasis on recent technologies that allow for large-scale and low-cost production.
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Causes and effects of N-terminal codon bias in bacterial genes.
Daniel B. Goodman,Daniel B. Goodman,Daniel B. Goodman,George M. Church,George M. Church,Sriram Kosuri +5 more
TL;DR: It is demonstrated that reduced RNA structure and not codon rarity itself is responsible for expression increases, which resolve controversies over the roles of N-terminal codon bias and suggest a straightforward method for optimizing heterologous gene expression in bacteria.