M
Mark Moosburner
Researcher at University of California, San Diego
Publications - 9
Citations - 2637
Mark Moosburner is an academic researcher from University of California, San Diego. The author has contributed to research in topics: Phaeodactylum tricornutum & Genome. The author has an hindex of 7, co-authored 8 publications receiving 2240 citations. Previous affiliations of Mark Moosburner include J. Craig Venter Institute & Harvard University.
Papers
More filters
Journal ArticleDOI
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Prashant Mali,John Aach,P. Benjamin Stranges,Kevin M. Esvelt,Mark Moosburner,Sriram Kosuri,Luhan Yang,George M. Church,George M. Church +8 more
TL;DR: This system is engineer to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).
Journal ArticleDOI
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
TL;DR: An in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ∼1,400 genomic loci is developed and provides a generalizable framework to study nucleic acid–nucleic acid interactions and biochemistry in high throughput.
Journal ArticleDOI
Design, synthesis, and testing toward a 57-codon genome
Nili Ostrov,Matthieu Landon,Matthieu Landon,Marc Güell,Marc Güell,Gleb Kuznetsov,Jun Teramoto,Jun Teramoto,Natalie Cervantes,Minerva Zhou,Kerry Singh,Michael G. Napolitano,Mark Moosburner,Ellen Shrock,Benjamin W. Pruitt,Nicholas Conway,Daniel B. Goodman,Daniel B. Goodman,Cameron L. Gardner,Gary Tyree,Alexandra Gonzales,Barry L. Wanner,Julie E. Norville,Marc J. Lajoie,George M. Church,George M. Church +25 more
TL;DR: Computational design, synthesis, and progress toward assembly of a 3.97-megabase, 57-codon Escherichia coli genome in which all 62,214 instances of seven codons were replaced with synonymous alternatives across all protein-coding genes are reported.
Journal ArticleDOI
Genetic tool development in marine protists: emerging model organisms for experimental cell biology
Drahomíra Faktorová,R. Ellen R. Nisbet,R. Ellen R. Nisbet,José A. Fernández Robledo,Elena Casacuberta,Lisa Sudek,Andrew E. Allen,Manuel Ares,Cristina Aresté,Cecilia Balestreri,Adrian C. Barbrook,Patrick Beardslee,Sara J. Bender,David S. Booth,François-Yves Bouget,Chris Bowler,Susana A. Breglia,Colin Brownlee,Gertraud Burger,Heriberto Cerutti,Rachele Cesaroni,Miguel Angel Chiurillo,Thomas E. Clemente,Duncan B. Coles,Jackie L. Collier,Elizabeth C. Cooney,Kathryn J. Coyne,Roberto Docampo,Christopher L. Dupont,Virginia P. Edgcomb,Elin Einarsson,Pia A. Elustondo,Fernán Federici,Verónica Freire-Benéitez,Nastasia J. Freyria,Kodai Fukuda,Paulo A. Garcia,Peter R. Girguis,Fatma Gomaa,Sebastian G. Gornik,Jian Guo,Jian Guo,Vladimír Hampl,Yutaka Hanawa,Esteban R. Haro-Contreras,Elisabeth Hehenberger,Andrea Highfield,Yoshihisa Hirakawa,Amanda Hopes,Christopher J. Howe,Ian Hu,Jorge Ibañez,Nicholas A.T. Irwin,Yuu Ishii,Natalia Ewa Janowicz,Adam C. Jones,Ambar Kachale,Konomi Fujimura-Kamada,Binnypreet Kaur,Jonathan Z. Kaye,Eleanna Kazana,Patrick J. Keeling,Nicole King,Lawrence A. Klobutcher,Noelia Lander,Imen Lassadi,Zhu-Hong Li,Senjie Lin,Jean Claude Lozano,Fulei Luan,Shinichiro Maruyama,Tamara Matute,Cristina Miceli,Jun Minagawa,Jun Minagawa,Mark Moosburner,Sebastián R. Najle,Sebastián R. Najle,Deepak Nanjappa,Isabel C. Nimmo,Luke M. Noble,Luke M. Noble,Anna M. G. Novák Vanclová,Mariusz Nowacki,Isaac Núñez,Arnab Pain,Arnab Pain,Angela Piersanti,Sandra Pucciarelli,Jan Pyrih,Jan Pyrih,Joshua S. Rest,Mariana Rius,Deborah L. Robertson,Albane Ruaud,Albane Ruaud,Iñaki Ruiz-Trillo,Iñaki Ruiz-Trillo,Monika Abedin Sigg,Pamela A. Silver,Claudio H. Slamovits,G. Jason Smith,Brittany N. Sprecher,Rowena Stern,Estienne C. Swart,Estienne C. Swart,Anastasios D. Tsaousis,Lev Tsypin,Lev Tsypin,Aaron P. Turkewitz,Jernej Turnšek,Matus Valach,Valérie Vergé,Peter von Dassow,Tobias von der Haar,Ross F. Waller,Lu Wang,Xiaoxue Wen,Glen L. Wheeler,April Woods,Huan Zhang,Thomas Mock,Alexandra Z. Worden,Julius Lukeš +123 more
TL;DR: The development of genetic tools in a range of protists primarily from marine environments are reported on, providing a roadmap for developing genetically tractable organisms.
Journal ArticleDOI
Reduction-dependent siderophore assimilation in a model pennate diatom.
Tyler H. Coale,Mark Moosburner,Mark Moosburner,Aleš Horák,Miroslav Oborník,Miroslav Oborník,Katherine A. Barbeau,Andrew E. Allen +7 more
TL;DR: Reverse genetic techniques are used to target putative iron-acquisition genes in the model pennate diatom Phaeodactylum tricornutum to confirm independent iron- Acquisition pathways in diatoms and characterize their preferred substrates, and to better predict the influence of iron speciation on taxa-specific iron bioavailability.