S
Stefan Höning
Researcher at University of Cologne
Publications - 62
Citations - 5333
Stefan Höning is an academic researcher from University of Cologne. The author has contributed to research in topics: Clathrin & Endosome. The author has an hindex of 36, co-authored 59 publications receiving 4930 citations. Previous affiliations of Stefan Höning include Max Planck Society & University of Göttingen.
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Journal ArticleDOI
A Large-Scale Conformational Change Couples Membrane Recruitment to Cargo Binding in the AP2 Clathrin Adaptor Complex
Lauren P. Jackson,Bernard T. Kelly,Airlie J. McCoy,Thomas Gaffry,Leo C. James,Brett M. Collins,Stefan Höning,Philip R. Evans,David J. Owen +8 more
TL;DR: Using a range of biophysical techniques, it is shown that the endocytic cargo binding of AP2 is driven by its interaction with PtdIns4,5P2-containing membranes.
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A di-leucine-based motif in the cytoplasmic tail of LIMP-II and tyrosinase mediates selective binding of AP-3
TL;DR: Monitoring surface plasmon resonance observed that AP‐3 is able to interact with the tail of the lysosomal integral membrane protein LIMP‐II and that this binding depends on a DEXXXLI sequence in the LIMP-II tail, which points to a function of AP‐ 3 in intracellular sorting to lysoomes and melanosomes of a subset of cargo proteins via di‐leucine‐based sorting motifs.
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A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex
Bernard T. Kelly,Airlie J. McCoy,Kira Späte,Sharon E. Miller,Philip R. Evans,Stefan Höning,David J. Owen +6 more
TL;DR: In this paper, the crystal structure of AP2 in a complex with the diceucine motif of a cargo protein was presented, and the mechanism of cargo-adaptor recognition was revealed.
Journal ArticleDOI
Phosphatidylinositol-(4,5)-bisphosphate regulates sorting signal recognition by the clathrin-associated adaptor complex AP2.
Stefan Höning,Doris Ricotta,Michael Krauss,Kira Späte,Barbara Spolaore,Alison M. Motley,Margaret S. Robinson,Carol V. Robinson,Volker Haucke,David J. Owen +9 more
TL;DR: It is demonstrated that recognition of Yxxvarphi and acidic dileucine motifs is dependent on corecognition with PtdIns4,5P(2), explaining the selective recruitment of AP2 to the plasma membrane.
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Phosphorylation of the AP2 μ subunit by AAK1 mediates high affinity binding to membrane protein sorting signals
TL;DR: It is proposed that phosphorylation of the AP2 μ2 subunit by AAK1 ensures high affinity binding of AP2 to sorting signals of cargo membrane proteins during the initial steps of receptor-mediated endocytosis.