S
Stephanie I Stephens
Researcher at Gladstone Institutes
Publications - 2
Citations - 28
Stephanie I Stephens is an academic researcher from Gladstone Institutes. The author has contributed to research in topics: Respiratory virus & RNA. The author has an hindex of 1, co-authored 2 publications receiving 12 citations. Previous affiliations of Stephanie I Stephens include University of California, Berkeley.
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Direct detection of SARS-CoV-2 using CRISPR-Cas13a and a mobile phone
Parinaz Fozouni,Sungmin Son,María Díaz de León Derby,María Díaz de León Derby,Gavin J. Knott,Gavin J. Knott,Carley N. Gray,Carley N. Gray,Michael V. D’Ambrosio,Chunyu Zhao,Neil A. Switz,G. Renuka Kumar,G. Renuka Kumar,Stephanie I Stephens,Stephanie I Stephens,Daniela Boehm,Daniela Boehm,Chia-Lin Tsou,Chia-Lin Tsou,Jeffrey H Shu,Jeffrey H Shu,Abdul Bhuiya,Abdul Bhuiya,Max Armstrong,Andrew R. Harris,Jeannette M. Osterloh,Anke Meyer-Franke,Charles Langelier,Katherine S. Pollard,Emily D. Crawford,Andreas S. Puschnik,Maira Phelps,Amy Kistler,Joseph L. DeRisi,Jennifer A. Doudna,Daniel A. Fletcher,Melanie Ott,Melanie Ott +37 more
TL;DR: This work reports the development of an amplification-free CRISPR-Cas13a-based mobile phone assay for direct detection of SARS-CoV-2 from nasal swab RNA extracts that achieved ~100 copies/L sensitivity in under 30 minutes and accurately detected a set of positive clinical samples in under 5 minutes.
Posted ContentDOI
Sensitive and multiplexed RNA detection with Cas13 droplets and kinetic barcoding
Sungmin Son,Amy Lyden,Amy Lyden,Jeffrey H Shu,Stephanie I Stephens,Parinaz Fozouni,Gavin J. Knott,Gavin J. Knott,Dylan C. J. Smock,Tina Y. Liu,Daniela Boehm,Camille R. Simoneau,Renuka Kumar,Jennifer A. Doudna,Melanie Ott,Melanie Ott,Daniel A. Fletcher +16 more
TL;DR: In this article, a droplet-based Cas13a assay with kinetic barcoding was proposed to detect SARS-CoV-2 variants within a single droplet by tracking nuclease activity within individual droplets over time.