G
Gavin J. Knott
Researcher at University of California, Berkeley
Publications - 42
Citations - 4119
Gavin J. Knott is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: CRISPR & RNA. The author has an hindex of 17, co-authored 37 publications receiving 2181 citations. Previous affiliations of Gavin J. Knott include Discovery Institute & Arizona State University.
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Journal ArticleDOI
CRISPR-Cas guides the future of genetic engineering.
TL;DR: The basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies are described, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies.
Journal ArticleDOI
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy.
Parinaz Fozouni,Sungmin Son,María Díaz de León Derby,Gavin J. Knott,Gavin J. Knott,Carley N. Gray,Carley N. Gray,Michael V. D’Ambrosio,Chunyu Zhao,Neil A. Switz,G. Renuka Kumar,G. Renuka Kumar,Stephanie I. Stephens,Stephanie I. Stephens,Daniela Boehm,Daniela Boehm,Chia-Lin Tsou,Chia-Lin Tsou,Jeffrey Shu,Jeffrey Shu,Abdul Bhuiya,Maxim Armstrong,Andrew R. Harris,Pei Yi Chen,Pei Yi Chen,Jeannette M. Osterloh,Anke Meyer-Franke,Bastian Joehnk,Keith Walcott,Anita Sil,Charles Langelier,Katherine S. Pollard,Emily D. Crawford,Andreas S. Puschnik,Maira Phelps,Amy Kistler,Joseph L. DeRisi,Jennifer A. Doudna,Daniel A. Fletcher,Melanie Ott +39 more
TL;DR: This work reports the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope and has the potential to enable rapid, low-cost, point-of-care screening for Sars-Cov-2.
Journal ArticleDOI
CasX enzymes comprise a distinct family of RNA-guided genome editors
Jun-Jie Liu,Jun-Jie Liu,Jun-Jie Liu,Natalia Orlova,Benjamin L. Oakes,Enbo Ma,Hannah Spinner,Katherine Baney,Jonathan Chuck,Dan Tan,Gavin J. Knott,Lucas B. Harrington,Basem Al-Shayeb,Alexander J. Wagner,Julian Brötzmann,Brett T. Staahl,Kian Taylor,John J Desmarais,Eva Nogales,Jennifer A. Doudna +19 more
TL;DR: The underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR–CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage, are revealed.
Journal ArticleDOI
CRISPR-CasΦ from huge phages is a hypercompact genome editor.
Patrick Pausch,Basem Al-Shayeb,Ezra Bisom-Rapp,Connor A. Tsuchida,Connor A. Tsuchida,Zheng Li,Brady F. Cress,Gavin J. Knott,Gavin J. Knott,Steven E. Jacobsen,Steven E. Jacobsen,Jillian F. Banfield,Jennifer A. Doudna +12 more
TL;DR: A minimal functional CRISpr-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages, which provides a hypercompact addition to the genome-editing toolbox.
Journal ArticleDOI
Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles
Sven Hennig,Sven Hennig,Geraldine Kong,Taro Mannen,Agata Sadowska,Simon Kobelke,Amanda J. Blythe,Gavin J. Knott,K. Swaminathan Iyer,Diwei Ho,Estella A. Newcombe,Kana Hosoki,Naoki Goshima,Tetsuya Kawaguchi,Danny M. Hatters,Laura Trinkle-Mulcahy,Tetsuro Hirose,Charles S. Bond,Archa H. Fox,Archa H. Fox +19 more
TL;DR: Paraspeckles are mammalian subnuclear bodies built on a long noncoding RNA and are enriched in RNA binding proteins with prion-like domains; two of these proteins, RBM14 and FUS, use these domains to hold paraspeckle together.