Showing papers by "Susan Lindquist published in 1984"
TL;DR: Evidence that hsps protect cells from the toxic effects of anoxia, as well as heat, is provided and it is concluded that nuclear translocation of hsp70 is related to its function in protecting the organism from both forms of environmental stress.
390 citations
TL;DR: Analysis of RNAs isolated from the yeast Saccharomyces cerevisiae reveals a dramatic series of changes in protein coding sequences during sporulation, which are unique to sporulating cells.
Abstract: Analysis of RNAs isolated from the yeast Saccharomyces cerevisiae reveals a dramatic series of changes in protein coding sequences during sporulation. Shortly after transfer to sporulation medium, mRNAs for certain proteins are repressed while a broad array of mRNAs for other proteins is induced. Superimposed on this general increase in transcriptional activity is the very strong induction of a particular subset of heat shock mRNAs, the same subset that is induced during the normal course of oogenesis in Drosophila. At distinct times later in sporulation, two sets of abundant mRNAs are coordinately induced. Unlike the earlier changes in the message complement, these changes are unique to sporulating cells. As asci mature, one set of sporulation-specific RNAs is selectively degraded. The second set, as well as the broad array of mRNAs induced earlier in development, is retained in a highly stable and fully translatable form.
94 citations
TL;DR: The results indicate that a single protein can have multiple interactions with cellular components, and form the basis for future studies of these interactions by in vitro mutagenesis and expression of the hsp70 gene.
Abstract: We transformed mouse L cells with a cloned Drosophila hsp70 gene and obtained cells with either heat-inducible or constitutively expressed copies of the gene. The distribution of hsp70 in these cells was examined by indirect immunofluorescence with monoclonal antibodies specific to the Drosophila protein. In constitutive cells, hsp70 was present in both cytoplasm and nucleus. After heat shock, the nuclear hsp70 was transiently concentrated in nucleoli, from which it had previously been excluded; the cytoplasmic hsp70 moved to a perinuclear location, a result consistent with it being associated with intermediate filaments of the cytoskeleton. The nucleolar migration took several hours and was partly inhibited by actinomycin D, but was independent of protein synthesis; it may reflect binding to newly-synthesized rRNA. Drosophila hsp70 was also expressed from replicating plasmids in monkey COS cells, and was found to be concentrated in nuclei even at low temperature. Migration to nucleoli occurred after heat shock. These results indicate that a single protein can have multiple interactions with cellular components, and form the basis for future studies of these interactions by in vitro mutagenesis and expression of the hsp70 gene.
31 citations
Journal Article•
TL;DR: When cells or whole organisms are exposed to temperatures slightly above their optimum for growth, they respond by synthesizing a small group of proteins, called the heat shock proteins (hsps), which help protect them from the toxic effects of heat.
Abstract: When cells or whole organisms are exposed to temperatures slightly above their optimum for growth, they respond by synthesizing a small group of proteins, called the heat shock proteins (hsps), which help protect them from the toxic effects of heat The same set of proteins can also be induced by a wide variety of other stresses including exposure to ethanol, heavy metal ions, and inhibitors of respiratory metabolism Their induction is apparently a very general reaction to adverse conditions (See Schlessinger, Ashburner & Tissieres, 1982, for review) This response is the most highly conserved genetic regulatory system known The proteins produced by fruit flies are homologous to those produced by enterobacteria, corn plants, slime moulds, yeasts, sea urchins, and humans (Bardwell & Craig, 1984; Key, Lin & Chen, 1981; Loomis & Wheeler, 1980; McAlister, Strausberg, Kulaga & Finkelstein, 1979; Giudice, Roccheri & Debernardo, 1980; Thomas, Welch, Matthews & Feramisco, 1982)
22 citations