Takayoshi Kuno

TL;DR: Radioactive phorbol 12,13-dibutyrate binding analysis indicated that a cysteine-rich zinc-finger-like sequence was essential for protein kinase C to bind phorbobol ester and that one of two sequences was sufficient for the phorbl ester binding.

TL;DR: An atrial natriuretic factor (ANF) receptor from rat lung was solubilized with Lubrol-PX and purified by sequential chromatographic steps on GTP-agarose, DEAE-Sephacel, phenyl-agarOSE, and wheat germ agglutinin-agaroses, suggesting that both ANF binding and guanylate cyclase activities reside in the same macromolecular complex.

TL;DR: Findings suggest that alterations in S2 receptors in the prefrontal cortex may reflect the disease process, per se, and that the increase in the number of D2 receptor sites in the caudate nucleus of schizophrenics is not due solely to neuroleptic medication.

TL;DR: Southern blot analysis of high molecular weight genomic DNA from rat liver indicated that the genes for the 70- and 82-kDa subunits are different, and Presumably both catalytic subunits must be present and interactive to permit synthesis of cyclic GMP and nitrovasodilator activation.

TL;DR: Data demonstrate that soluble guanylate cyclase purified from rat lung is a heterodimer composed of 82-000- and 70,000-dalton subunits with different primary structures.