Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung.
TLDR
Southern blot analysis of high molecular weight genomic DNA from rat liver indicated that the genes for the 70- and 82-kDa subunits are different, and Presumably both catalytic subunits must be present and interactive to permit synthesis of cyclic GMP and nitrovasodilator activation.About:
This article is published in Journal of Biological Chemistry.The article was published on 1990-10-05 and is currently open access. It has received 231 citations till now. The article focuses on the topics: Guanylate cyclase activity & GUCY1A2.read more
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Neuronal NADPH diaphorase is a nitric oxide synthase.
TL;DR: It is found that nitric oxide synthase activity and NAD PH diaphorase copurify to homogeneity and that both activities could be immunoprecipitated with an antibody recognizing neuronal NADPH diaphirase.
Journal Article
Guanylyl Cyclases and Signaling by Cyclic GMP
Kimberly A. Lucas,Giovanni Pitari,Giovanni Pitari,Shiva Kazerounian,Inez Ruiz-Stewart,Jason Y. Park,Stephanie Schulz,Kenneth P. Chepenik,Scott A. Waldman +8 more
TL;DR: Guanylyl cyclases are a family of enzymes that catalyze the conversion of GTP to cGMP as mentioned in this paper, and they are regulated by diverse extracellular agonists that include peptide hormones, bacterial toxins, and free radicals, as well as intracellular molecules such as calcium and adenine nucleotides.
Journal ArticleDOI
Guanylate cyclase and the ⋅NO/cGMP signaling pathway
TL;DR: This review will focus on the role of sGC in signaling, its relationship to the other nucleotide cyclases, and on what is known about sGC genetics, heme environment and catalysis.
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Crystal Structure of the Catalytic Domains of Adenylyl Cyclase in a Complex with Gsα·GTPγS
TL;DR: The crystal structure of a catalytically active form of adenylyl cyclase in a complex with its stimulatory heterotrimeric G protein α subunit (Gs α) and forskolin was determined to a resolution of 2.3 angstroms as discussed by the authors.
References
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Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
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Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Haim Aviv,Philip Leder +1 more
TL;DR: A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
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High-efficiency transformation of mammalian cells by plasmid DNA.
TL;DR: A simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells are described and linear DNA is almost inactive in mammalian cells.
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Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
TL;DR: The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
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Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.