Theresa J. Kokkat

TL;DR: It is shown that there is no significant difference between macromolecules extracted from blocks stored over 11-12 years, 5-7 years, or 1-2 years in comparison to the current year blocks.

TL;DR: The first version of the Standard PREanalytical Code (SPREC) was developed in 2009 by the International Society for Biological and Environmental Repositories (ISBER) Biospecimen Science Working Group to facilitate documentation and communication of the most important preanalytical quality parameters of different types of biospecimens used for research.

TL;DR: In the current era of minimally invasive techniques to obtain minimal amount of tissue for diagnostic and prognostic purposes, the use of commercial and lab made buffer on low weight MFPE scrapings obtained by Cellient® processor opens new possibilities for protein biomarker research.

TL;DR: This study investigated methanol-fixed paraffin-embedded (MFPE) cell blocks for protein yield employing the Cellient automated cell block system.

TL;DR: Results showed that in benign, malignant, and diseased tissues, high temperature thawing is effective in reducing enzymatic activity, which suggests that if tissue lysates are to be obtained from frozen tissues without the addition of inhibitors, high Temperature Thawing might have marked improvement in downstream non-enzymatic analyses of diseased and neoplastic tissues.