Thomas Raschle

TL;DR: This paper demonstrates that the combination of small nanodisc size, high deuteration levels of protein and lipids, and the use of advanced non-uniform NMR sampling methods enable the NMR resonance assignment as well as the high-resolution structure determination of polytopic membrane proteins in a detergent-free, near-native lipid bilayer setting.

TL;DR: It is demonstrated that Arabidopsis thaliana has two functional homologs of PDX1 and a single homolog ofPDX2, and it is reported that the single PDX2 homolog is essential for plant viability.

TL;DR: In this article, the authors compare the two independent pathways to the biosynthesis of vitamin B6 and reveal that the key biosynthetic enzymes of both pathways are, in fact, very similar both structurally and mechanistically.

TL;DR: The ability to study integral membrane proteins at atomic resolution with solution NMR in phospholipid bilayers, rather than in detergent micelles, offers exciting novel possibilities to approach the biophysical properties of membrane proteins under nondenaturing conditions, which makes this technology particular suitable for protein-protein interactions and other functional studies.

TL;DR: This report corroborates a recent report on the identification of the substrates of YaaD and provides unequivocal proof of the identity of the reaction product and reports on the detailed characterization of the inhibition of the glutaminase domain, and thus PLP synthesis, by the glutamine analog acivicin.