T
Todd A. Aguilera
Researcher at University of Texas Southwestern Medical Center
Publications - 58
Citations - 3557
Todd A. Aguilera is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: Cancer & Radiation therapy. The author has an hindex of 19, co-authored 47 publications receiving 3101 citations. Previous affiliations of Todd A. Aguilera include University of California, San Diego & Stanford University.
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Journal ArticleDOI
Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome.
Xiaokun Shu,Antoine Royant,Michael Z. Lin,Todd A. Aguilera,Varda Lev-Ram,Paul Steinbach,Roger Y. Tsien +6 more
TL;DR: It is shown that a bacteriophytochrome from Deinococcus radiodurans can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M−1 cm−1; and quantum yield of 0.07.
Journal ArticleDOI
Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases
Emilia S. Olson,Tao Jiang,Todd A. Aguilera,Quyen T. Nguyen,Lesley G. Ellies,Miriam Scadeng,Roger Y. Tsien +6 more
TL;DR: In vivo visualization of matrix metalloproteinase activities by MRI and fluorescence of dendrimeric nanoparticles coated with activatable cell penetrating peptides, labeled with Cy5, gadolinium, or both shows that uptake via this mechanism is comparable to that of other near infrared protease sensors, with the added advantage that the approach is translatable to MRI.
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Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival
Quyen T. Nguyen,Emilia S. Olson,Todd A. Aguilera,Tao Jiang,Miriam Scadeng,Lesley G. Ellies,Roger Y. Tsien +6 more
TL;DR: A method to visualize tumors during surgery using activatable cell-penetrating peptides (ACPPs), in which the fluorescently labeled, polycationic cell-peptide (CPP) is coupled via a cleavable linker to a neutralizing peptide, resulting in improved precision of tumor resection.
Journal ArticleDOI
In vivo characterization of activatable cell penetrating peptides for targeting protease activity in cancer
Emilia S. Olson,Todd A. Aguilera,Tao Jiang,Lesley G. Ellies,Quyen T. Nguyen,Edmund H. Wong,Larry A. Gross,Roger Y. Tsien +7 more
TL;DR: An approach that should generally deliver imaging agents and chemotherapeutics to sites of invasion, tumor-promoting inflammation, and metastasis is validated, and accumulation is strongest at the tumor-stromal interface in primary tumors and associated metastases, indicating better spatial resolution than other currently available MMP-cleavable probes.
Journal ArticleDOI
Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals
Michael Z. Lin,Michael R. McKeown,Ho Leung Ng,Todd A. Aguilera,Nathan C. Shaner,Robert E. Campbell,Stephen R. Adams,Larry A. Gross,Wendy Ma,Tom Alber,Roger Y. Tsien +10 more
TL;DR: The evolution of far-red fluorescent proteins with peak excitation at 600 nm or above are reported, and the brightest one, Neptune, performs well in imaging deep tissues in living mice and reveals a novel mechanism for red-shifting involving the acquisition of a new hydrogen bond with the acylimine region of the chromophore.