T
Toine ten Broeke
Researcher at Utrecht University
Publications - 29
Citations - 1758
Toine ten Broeke is an academic researcher from Utrecht University. The author has contributed to research in topics: Antibody & Endosome. The author has an hindex of 16, co-authored 27 publications receiving 1456 citations. Previous affiliations of Toine ten Broeke include University Medical Center Utrecht.
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Journal ArticleDOI
MHC II In dendritic cells is targeted to lysosomes or T cell-induced exosomes via distinct multivesicular body pathways
Sonja I. Buschow,Esther N. M. Nolte-‘t Hoen,Guillaume van Niel,Maaike S. Pols,Toine ten Broeke,Marjolein Lauwen,Ferry Ossendorp,Cornelis Johannes Maria Melief,Graça Raposo,Richard Wubbolts,Marca H. M. Wauben,Willem Stoorvogel +11 more
TL;DR: Evidence is provided for an alternative MVB sorting mechanism for MHC II in antigen‐loaded DCs, which is triggered by cognately interacting antigen‐specific CD4+ T cells, and data indicate two distinct MVB pathways: one for lysosomal targeting and the other for exosome secretion.
Journal ArticleDOI
Differential MHC class II synthesis and ubiquitination confers distinct antigen-presenting properties on conventional and plasmacytoid dendritic cells
Louise J. Young,Nicholas S. Wilson,Petra Schnorrer,Petra Schnorrer,Anna I Proietto,Anna I Proietto,Toine ten Broeke,Yohei Matsuki,Adele M. Mount,Adele M. Mount,Gabrielle T. Belz,Gabrielle T. Belz,Meredith O'Keeffe,Meredith O'Keeffe,Mari Ohmura-Hoshino,Satoshi Ishido,Willem Stoorvogel,William R. Heath,William R. Heath,William R. Heath,Ken Shortman,Jose A Villadangos,Jose A Villadangos,Jose A Villadangos +23 more
TL;DR: It is shown that unlike cDCs, pDCs continued to synthesize major histocompatibility complex (MHC) class II molecules and the MHC class II ubiquitin ligase MARCH1 long after activation, enabling pDCS to continuously present endogenous viral antigens in their activated state.
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Identification of a novel ubiquitin conjugation motif, required for ligand-induced internalization of the growth hormone receptor.
TL;DR: Evidence is presented that a specific domain of the GHR regulates receptor endocytosis via the ubiquitin conjugation system, which consists of the amino acid sequence DSWVEFIELD and is homologous to sequences in other proteins, several of which are known to be ubiquitinated.
Journal ArticleDOI
Dendritic cells regulate exposure of MHC class II at their plasma membrane by oligoubiquitination.
Guillaume van Niel,Richard Wubbolts,Toine ten Broeke,Sonja I. Buschow,Ferry Ossendorp,Cornelis J. M. Melief,Graça Raposo,Bas W.M. van Balkom,Willem Stoorvogel +8 more
TL;DR: It is demonstrated that in immature DCs, the beta-chain of MHC class II was oligoubiquitinated after proteolytic processing of the associated invariant chain in endosomes and that this modification was required for efficient endocytosis and sorting into luminal vesicles of multivesicular bodies.
Journal ArticleDOI
Endocytosed Transferrin Receptors Recycle via Distinct Dynamin and Phosphatidylinositol 3-Kinase-dependent Pathways
Ellen M. van Dam,Toine ten Broeke,Karen G. J. J. Jansen,Patricia P. Spijkers,Willem Stoorvogel +4 more
TL;DR: Two distinct recycling pathways are shown, one of which involves transfer from early endosomes to recycling endOSomes, from where clathrin/dynamin-coated vesicles provide for further transport, whereas the other route bypasses recycling endosome and requires phosphatidylinositol 3-kinase activity.