Showing papers by "Tomaso Patarnello published in 2005"
TL;DR: While D. puntazzo presented a marked genetic divergence between the Atlantic and Mediterranean samples, D. sargus showed little intraspecific differentiation, interpreted as the consequence of differences in the history of the two species such as fluctuations in the effective population size due to bottlenecks and expansions, possibly combined with present-day differences in levels of gene flow.
95 citations
TL;DR: The genetic structure, as revealed by analysis of population samples collected at the same site in consec- utive years, was stable during that sampling period, and revealed the existence of 4 geneti- cally and geographically distinct gene pools of M. norvegica.
Abstract: Meganyctiphanes norvegica (M Sars 1856), the northern krill, is the largest and most abundant euphausiid species in the northern hemisphere, where it represents a key component of many pelagic communities Although planktonic, krill could be considered a nektonic organism, as it is capable of active movements This behaviour may be adaptive, because it allows these organisms to maintain their geographic position, leading to stable population structure, despite being continu- ously exposed to the heterogeneous oceanic conditions By means of single-strand conformation polymorphism and DNA sequencing, we analyzed allelic variation of the Subunit 1 of NADH dehy- drogenase mtDNA locus in 23 populations of the northern krill Meganyctiphanes norvegica, from 15 locations spanning the distribution range of the species Analysis of the data indicated that the genetic structure, as revealed by analysis of population samples collected at the same site in consec- utive years, was stable during that sampling period Our results revealed the existence of 4 geneti- cally and geographically distinct gene pools of M norvegica, 2 occurring in the NE Atlantic ('north- ern' NE Atlantic and 'southern' NE Atlantic), 1 in the NW Atlantic, and 1 in the Mediterranean (Ligurian) Sea
57 citations
TL;DR: Sexual pheromone traps are commonly used to monitor populations of the pine processionary moth, Thaumetopoea pityocampa, assuming that trapped males are representative of the breeding population.
Abstract: 1 Sexual pheromone traps are commonly used to monitor populations of the pine processionary moth, Thaumetopoea pityocampa, assuming that trapped males are representative of the breeding population.
2 For seven Italian populations, mitochondrial haplotypes (COI and COII) of adult males caught in traps were compared with those of larvae obtained from egg batches laid in the same year, to test whether the males trapped were representative of the local populations.
3 The distribution of haplotype frequencies revealed substantial homogeneity between adult males and larvae samples from the same population, except for Aosta Ruines Verres, a population recently expanding in the south-western Alps. In this case, the results suggested that trapped males were recruited over a wider area than local moths because haplotype diversity was higher than that of larvae.
4 A further analysis of this population using nuclear markers (AFLP) confirmed that adults, collected in pheromone traps, were genetically different from the larvae emerging in the same stand.
5 In conclusion, the assumption that trapped males are representative of breeding populations was confirmed for core populations, but has not been verified for the recently established population of Aosta Ruines Verres. This should encourage discussion with respect to the reliability of pheromone traps in monitoring programmes of the pest, especially at the range's edge.
21 citations
TL;DR: Positive results were obtained on fish with clear signs of the disease as well as on challenged, but asymptomatic, fish, suggesting the PCR-RFLP method might provide a useful tool for both prevention and rapid diagnosis of fish pasteurellosis.
Abstract: Fish pasteurellosis is an infectious disease that affects several teleost species living in temperate marine waters. The pathogen responsible, Photobacterium damselae subspecies piscicida, shows high genetic similarity with P. damselae subsp. damselae, making subspecies discrimination extremely laborious. Here we report for the first time a PCR-RFLP method for the identification of P. damselae subsp. piscicida without prior isolation in pure culture. Genomic sequence information was obtained through cloning and sequencing of RAPD products. Two P. damselae-specific primer pairs were developed and tested on 17 strains of P. damselae subsp. piscicida, 10 strains of P. damselae subsp. damselae, and 6 closely related control species. High sensitivity was achieved in PCR amplification on serially diluted samples (<180 fg of pure bacterial DNA or <10 fg, depending on the amplified fragment). Restriction analysis of PCR products showed a unique digestion profile for all P. damselae subsp. piscicida strains. The same PCR-RFLP method was implemented on total DNA samples extracted from experimentally infected sea bream and sea bass. Positive results were obtained on fish with clear signs of the disease as well as on challenged, but asymptomatic, fish. The method presented here might provide a useful tool for both prevention and rapid diagnosis of fish pasteurellosis.
19 citations
TL;DR: The isolation and sequencing of feline estrogen receptor alpha mRNA showed three alternative 5' untranslated exons and a common transcript of 6183 base pairs, and a highly conserved sequence block was found in the 3'-UTR corresponding to a putative regulatory element for mRNA stability.
13 citations