T
Toshiyuki Takagi
Researcher at Tokyo Institute of Technology
Publications - 8
Citations - 2118
Toshiyuki Takagi is an academic researcher from Tokyo Institute of Technology. The author has contributed to research in topics: DSIF & Transcription (biology). The author has an hindex of 6, co-authored 8 publications receiving 2010 citations.
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Journal ArticleDOI
NELF, a Multisubunit Complex Containing RD, Cooperates with DSIF to Repress RNA Polymerase II Elongation
Yuki Yamaguchi,Toshiyuki Takagi,Tadashi Wada,Keiichi Yano,Akiko Furuya,Seiji Sugimoto,Jun Hasegawa,Hiroshi Handa +7 more
TL;DR: The identification and purification from HeLa nuclear extract of a third protein factor required for DRB-sensitive transcription, termed negative elongation factor (NELF), cooperates with DSIF and strongly represses pol II elongation.
Journal ArticleDOI
Dsif, a novel transcription elongation factor that regulates rna polymerase ii processivity, is composed of human spt4 and spt5 homologs
Tadashi Wada,Toshiyuki Takagi,Yuki Yamaguchi,Anwarul Ferdous,Takeshi Imai,Susumu Hirose,Seiji Sugimoto,Keiichi Yano,Grant A. Hartzog,Fred Winston,Stephen Buratowski,Hiroshi Handa +11 more
TL;DR: The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo.
Journal ArticleDOI
Evidence that p-tefb alleviates the negative effect of dsif on rna polymerase ii-dependent transcription in vitro
TL;DR: A molecular basis for DRB action is revealed and suggests that P‐TEFb stimulates elongation by alleviating the negative action of DSIF, which binds to RNA Pol II and may directly regulate elongation.
Journal ArticleDOI
Structure and function of the human transcription elongation factor DSIF.
TL;DR: In this paper, the structure and function of DSIF p160 is characterized and a mutant that represses DSIF activity in a dominant negative manner is identified and used to demonstrate that DSIF represses transcription from various promoters in vivo.
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Casein kinase II interacts with the bZIP domains of several transcription factors
TL;DR: It is demonstrated that CKII binds through its catalytic alpha and alpha' subunits to the basic leucine zipper DNA-binding domains of many transcription factors, including ATF1, and efficiently phosphorylates its substrate, which is bound to the same DNA molecule.