F
Fred Winston
Researcher at Harvard University
Publications - 154
Citations - 24979
Fred Winston is an academic researcher from Harvard University. The author has contributed to research in topics: Gene & Transcription (biology). The author has an hindex of 74, co-authored 150 publications receiving 23984 citations. Previous affiliations of Fred Winston include Massachusetts Institute of Technology & University of Iowa.
Papers
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Journal ArticleDOI
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
Charles S. Hoffman,Fred Winston +1 more
TL;DR: To release plasmid DNA for the transformation of Escherichia coli, cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform.
Book
Methods in Yeast Genetics: A Laboratory Course Manual
TL;DR: An intensive course in yeast genetics has been held at Cold Spring Harbor Laboratory for some years, and the course manual reflects its content and scope as discussed by the authors, with a focus on yeast genetics.
Journal ArticleDOI
Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
Patrick A. Grant,Laura J. Duggan,Jacques Côté,S M Roberts,James E. Brownell,Reyes Candau,Reiko Ohba,Tom Owen-Hughes,C D Allis,Fred Winston,Shelley L. Berger,Jerry L. Workman +11 more
TL;DR: The function of Gcn5 as a hist one acetyltransferase within the Ada and SAGA adaptor complexes indicates the importance of histone acetylation during steps in transcription activation mediated by interactions with transcription activators and general transcription factors.
Journal ArticleDOI
Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
TL;DR: A set of GAL2+ yeast strains that are isogenic to strain S288C have been constructed and contain non‐reverting mutations in genes commonly used for selection for recombinant plasmids.
Journal ArticleDOI
Dsif, a novel transcription elongation factor that regulates rna polymerase ii processivity, is composed of human spt4 and spt5 homologs
Tadashi Wada,Toshiyuki Takagi,Yuki Yamaguchi,Anwarul Ferdous,Takeshi Imai,Susumu Hirose,Seiji Sugimoto,Keiichi Yano,Grant A. Hartzog,Fred Winston,Stephen Buratowski,Hiroshi Handa +11 more
TL;DR: The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo.