T
Tsutomu Nishihara
Researcher at Osaka University
Publications - 140
Citations - 3719
Tsutomu Nishihara is an academic researcher from Osaka University. The author has contributed to research in topics: Bacillus megaterium & Gene. The author has an hindex of 33, co-authored 140 publications receiving 3573 citations. Previous affiliations of Tsutomu Nishihara include Tokai Gakuen University & Hyogo University of Health Sciences.
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Journal ArticleDOI
Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TL;DR: A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.
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New Screening Methods for Chemicals with Hormonal Activities Using Interaction of Nuclear Hormone Receptor with Coactivator
Jun-ichi Nishikawa,Koichi Saito,Koichi Saito,Jun Goto,Fumi Dakeyama,Masatoshi Matsuo,Masatoshi Matsuo,Tsutomu Nishihara +7 more
TL;DR: It is demonstrated that the measurement of interaction between steroid hormone receptor and coactivator serves as a useful tool for identifying chemicals that interact with steroid receptors.
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DNA Binding Specificity of the CCAAT/Enhancer-binding Protein Transcription Factor Family
TL;DR: With small variations, these C/EBP family members showed similar sequence preferences, and the consensus sequence was identified as RTTGCGYAAY (R = A or G, and Y = C or T), and the phosphorylation of C/ EBP by casein kinase II increased the binding activity, but did not affect the binding specificity.
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Trialkyltin Compounds Bind Retinoid X Receptor to Alter Human Placental Endocrine Functions
Tsuyoshi Nakanishi,Jun-ichi Nishikawa,Youhei Hiromori,Hideaki Yokoyama,Mihoko Koyanagi,Shinri Takasuga,Jun-ichi Ishizaki,Mai Watanabe,Shun-ichi Isa,Naoki Utoguchi,Norio Itoh,Yutaka Kohno,Tsutomu Nishihara,Keiichi Tanaka +13 more
TL;DR: The results suggest that activation of RXR may be a novel mechanism by which trialkyltins alter human endocrine functions through RXR-dependent signaling pathways.
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Estrogenic Activities of UV Stabilizers Used in Food Contact Plastics and Benzophenone Derivatives Tested by the Yeast Two-Hybrid Assay
Yoko Kawamura,Yuko Ogawa,Tetsuji Nishimura,Yutaka Kikuchi,Jun-ichi Nishikawa,Tsutomu Nishihara,Ken-ichi Tanamoto +6 more
TL;DR: In this paper, the effect of the hydroxyl group in the phenol moiety were in order of 4- > 3- > 2-position, while the chloro group added at the 2 position of the 4-hydroxylated benzene ring enhanced the activity.