Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Concentrations of parabens in human breast tumours
TL;DR: Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin‐layer chromatography and this should open the way technically for more detailed information to be obtained on body burdens of paraben and in particular whether body burdens are different in cancer from those in normal tissues.
Journal ArticleDOI
Removal mechanisms for endocrine disrupting compounds (EDCs) in wastewater treatment — physical means, biodegradation, and chemical advanced oxidation: A review
TL;DR: Removal of EDCs from three aspects is reviewed, that is, physical means, biodegradation, and chemical advanced oxidation (CAO).
Journal ArticleDOI
Comparative study of the endocrine-disrupting activity of bisphenol A and 19 related compounds.
Shigeyuki Kitamura,Tomoharu Suzuki,Seigo Sanoh,Ryuki Kohta,Norimasa Jinno,Kazumi Sugihara,Shin'ichi Yoshihara,Nariaki Fujimoto,Hiromitsu Watanabe,Shigeru Ohta +9 more
TL;DR: The results suggest that the 4-hydroxyl group of the A- phenyl ring and the B-phenyl ring of BPA derivatives are required for these hormonal activities, and substituents at the 3,5-positions of the phenyl rings and the bridging alkyl moiety markedly influence the activities.
Journal ArticleDOI
Paraben esters: review of recent studies of endocrine toxicity, absorption, esterase and human exposure, and discussion of potential human health risks.
TL;DR: There is a need to carry out detailed evaluation of the potential for parabens, together with other oestrogenic and genotoxic co‐formulants of bodycare cosmetics, to increase female breast cancer incidence, to interfere with male reproductive functions and to influence development of malignant melanoma.
Journal ArticleDOI
NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A
Robert E. Chapin,Jane Adams,Kim Boekelheide,L. Earl Gray,Simon W. Hayward,Peter S.J. Lees,Barry S. McIntyre,Kenneth M. Portier,Teresa M. Schnorr,Sherry G. Selevan,John G. Vandenbergh,Susan Woskie +11 more
TL;DR: This research presents a meta-analyses of the immune system’s response to exposure to radiation and shows clear patterns of decline in the immune systems of men and women aged 65 and over.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.